Subsequently, the DNA fragment found above is used to determine its size, this is done through virtual digestion, where the restriction enzymes BamHI and PsiI are used.
3.3.1. Virtual Digestion
Cutting sites are established by the restriction enzymes BamHI and PsiI, for this process it should benoted that restriction enzymes are only functional under certain temperature conditions, pH, buffer.
NOTE: The selected enzymes are from NEB (New England Biolabs), for which the provider reports the following conditions of functionality in Figure \ref{971780}.