When is open the vector map within the Benchling online tool, the insertion of the codon sequence within the plasmid is located in the region followed by the promoter and previous of the MCS. For the selection of restriction enzymes, we work with the cutting enzymes listed by NEB within the Benchling tool.
Where it is observed that the plasmid promoter is found the enzyme BamHI which makes a cohesive cut, the other restriction enzyme selected is PsiI, this is located at the end of the area where the gene is introduced. The information contained between the two plasmid enzymes (BamHI and PsiI) is replaced to clone there the AND sequence of the modified Ovalbumin protein, the procedure consists of disposition of these enzymes at the beginning and end of the optimized sequence of codons for ovalbumin. When cutting the sequence with BamHI and PsiI, the entire sequence is taken from BamHI to the site where it cuts the PsiI enzyme all this based on the leading strand of the AND chain, simultaneously and based on the complementary thread is taken what includes between the enzyme PsiI to the cutting of the enzyme  BamHI, the product of doing this is the sequence which is subsequently inserted into the plasmid pPIC9K  with the help of the ligase enzyme.