3.1.1 Reverse translation
Starting from the modified sequence of amino acids for the Ovalbumin protein (Figure \ref{175219}) and by means of the online tool Sequence Manipulation Suite (www.bioinformatics.org) reversed translation of amino acid sequence was performed. Simultaneously in the same interface of Sequence Manipulation Suite you enter the most likely s codon usage table for our Pichia Pastoris agency (this due to the snout bias) illustrated below.