After obtaining the sequence of codons of the ovalbumin protein inserted in the plasmid pPIC9K gives way to the realization of optimization for the sequence of codons for which we have the Benchling tool, to carry out this process taking into account that the host organism corresponds to Pichia Pastoris, it also generates the elimination of sequences of restriction enzymes that are not necessary for the case (the enzymes of restrictions must be protected so as not to be removed), and defining the GC content between 0.33 – 0.66 this in order to have greater stability in the sequence to be cloned. The results are reported in Figure \ref{433716}.   
3.3. Design for gene cloning in pPIC9K plasmid for Pichia Pastoris
After obtaining the optimized la codon sequence for Ovalbumin and through the Benchiling's online tool, the step is to perform cloning, this cloning is executed with a vector corresponding to the plasmid pPIC9K.  For this, the first to consider is to establish the coding gene of the protein (as well as the single cutting sites corresponding to the Enzymes BamHI and PsiI). The gene to be sequence for the modified ovalbumin corresponds to the optimized sequence of codons subsequently developed (see Figure \ref{433716}). This is done by considering that what is going to occur corresponds to the optimized sequence of codons plus the restriction site (because the restriction site allows us to "cut" and "paste" our sequence into our pPIC9K plasmid).Restriction enzymes cut into DNA, preferably restriction enzymes are not left at the ends of the chain, so it's convenient to additionally take a number of nucleotides before BamHI and after PsiI, then the sequence to be synthesized (including the optimized sequence of  codons for ovalbumin + cutting sites + additional nucleotides) corresponds to the one shown in Figure \ref{920475}.