To select the plasmid to use first, the vector expression for Pichia Pastoris is searched using the EMBL online tool (www.embl.de). Due to the great use that has commercial mind pPIC9K (with promoter AOX1) was selected as a vector to carry out cloning in Pichia Pastoris \cite{invitrogen2010}. For the download of the pPIC9K plasmid map the SnapGene software (www.snapgene.com), this map was of great importance because it made the relevant modifications for the cloning of the protein in the Benchling tool.