With this clear, the next step was to obtainer the sequence of AND encoding the sequence of inserted amino acids (enhanced sequence of amino acids for Ovalbumin), that is, the sequence of AND to be cloned without optimization of codons for the Ovalbumin of interest (see Figure reported in results and discussion).
3.2 Optimized sequence of codons for the Pichia Pastoris playback platform
Through the online application Benchling (www.benchling.comdevelops the project for the cloning of the protein of interest, for this the optimized sequence of codons will be inserted into the Benchling tool.
3.2.1. Plasmid selection