Column 1 of Figure \ref{272503} developed the electrophoresis for pPIC9K in its original state, where a higher band corresponding to a size of 9.1 kb of the acquired plasmid is observed. On the other hand in column 2 of Figure \ref{272503}, two fragments are observed, the smaller fragment corresponds to the ovalbumin protein, which is the gene to be cloned with a size of 1.2 kb, while the other fragment corresponds to the plasmid pPIC9K of 9.1 kb. By linking the gene to be cloned (lower band column 2) and plasmid (upper band column 1) the digestion observed in column 3 is obtained with a band size of 10.2 kb, which validates the insertion of the ovalbumin protein within the plasmid pPIC9K.   
5. Conclusions
The sequence of codons optimized using the computational tools allows to guarantee the stabilization of the sequence of this gene for the execution of its cloning in Pichia Pastoris with pPIC9K as a cloning vector (under the functionality conditions of the BamHI and PSiI restriction enzymes). In addition, through electrophoresis it is possible to validate the correct insertion of the optimized sequence to be cloned within the plasmid, for its subsequent downstream production strategy.