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Allergen modification of the ovalbumin as possible method to minimize the egg allergenic reaction into the human organism and its inclusion in a food product     
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  • Yiovann Alirio Arce Portilla,
  • Felipe Rojas,
  • Natalia Erazo Clavijo,
  • Jessica Agresott,
  • Daniela Guáqueta
Yiovann Alirio Arce Portilla
University of Los Andes (Uniandes)
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Felipe Rojas
University of Los Andes (Uniandes)
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Natalia Erazo Clavijo
University of Los Andes (Uniandes)
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Jessica Agresott
University of Los Andes (Uniandes)
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Daniela Guáqueta
University of Los Andes (Uniandes)
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Abstract

The production strategy for enhanced ovalbumin protein was realized based on the allergenic properties described in the previous delivery. As a production agency Pichia Pastoris was established which is a highly used eukaryotic organism for the mass production of proteins and pPIC9K was chosen as the cloning vector of the sequence to be synthesized. For the protein production strategy, the computer tools available on the web; sequence Manipulation Suite (www.bioinformatics.org) were used for reverse translation of the protein; Codon Usage Database (www.kazusa.or.jp) for the most likely codon usage table in Pichia PastorisEMBL  (www.embl.de) for vector selection of interest; SnapGene (www.snapgene.com) as a search repository for plasmid mapping and Benchling (www.benchling.com) for the gene cloning process to be synthesized with the optimized codon sequence for our ovalbumin protein.