RNA-seq and Assembly
In total, the 493.15 million reads were produced from the transcriptome sequencing (RNA-seq) of eight samples that includes one sample from each four genotypes under stress and control conditions, with about 58.44 to 65.38 million reads per sample (Table 1). Reads with adapter contamination and low quality base (≤Q20) were trimmed using Trimmomatic (Bolger et al. , 2014) and Fast-QCbox (Katta et al. 2015). As a result of this screening, we obtained a total of 465.80 million (~94%) high quality reads, and total of 440.3 million reads (94.55%) were mapped on to soybean reference genome (v1.0) (Table 1; Schmutz et al. , 2010).