qRT-PCR Validation
In order to validate the reliability and reproducibility of RNA-seq results, we selected above ten predicated candidate genes plus three random DEGs related to stress response for qRT-PCR validation (Table S1 ). By comparing qRT-PCR and RNAseq data, the relative expression of qRT-PCR analysis was also converted into FC by base 2 for consistency with the RNA-Seq data. These 12 genes include both up-regulated and down-regulated genes (Table S14 ). The results of qRT-PCR analysis of all these 12 genes showed consistency with the RNAseq data (Fig. 10). Overall, the results of qRT-PCR analysis showed the same expression trends as that of RNA-Seq, indicating the reliability of transcriptomic data used for the analysis in the present study.