Sample collection
The skin of three groups of discus females in the
parental females (their child swim
for one week, P_S (1,2,3) ) and the skin of three groups of discus
females in the non- parental females (the fifth day after swimming for
two weeks, NP_S (1,2,3) ) were put into a centrifuge tube and
temporarily frozen in liquid nitrogen. After that, they were put into a
refrigerator at -80℃ for long-term preservation and waiting for
treatment.
The brain of three groups of discus females in the parental females
(their child swim for one week, P_B (1,2,3) ) and the brain of three
groups of discus females in the non- parental females (the fifth day
after swimming for two weeks, NP_B (1,2,3) ) were put into a centrifuge
tube and temporarily frozen in liquid nitrogen. After that, they were
put into a refrigerator at -80℃ for long-term preservation and waiting
for treatment.