DEGs in the skin and brain
Through KEGG enrichment analysis of differentially expressed genes in skin (Fig 5 A), 7 skin feeding related pathways were screened: Arachidonic acid metabolism pathway, Adhesion pathway, Apoptosis, Steroid biosynthesis, Tuberculosis, P53 signaling pathway, serotonergic synapse. The genes in the pathway were analyzed and ten significantly different genes associated with lactation were selected for validation. These DEGs included JUNB, MRC, DPP3, CASP3, PPID, ITGA11, ALOXE3, HBE, PTPRJ, GALE. Identically, through KEGG enrichment analysis of differentially expressed genes in brain (Fig 5 B), 20 lactation related pathways were screened: Estrogen signaling pathways, Inflammatory medlator regulation of TRP channels, non-small cell lung cancer, Vascular smooth muscle contraction, GnRH signaling pathway , TypeⅡdiabetes mellitus, Neurotrophic signaling pathway, NOD-like receptor signaling pathway, Taste transduction, circadian rhythm, Jak-STAT signaling pathway, insulin resistance, Fc gamma R-mediated phagocytosis, Serotonergic synapses, Autophagy-animal, Small cell lung cancer, Pathways in cancer, AGE-RAGE signaling pathway in diabetic complications, Cytokin-cytokine receptor interaction. These DEGs included PRKCD, H1-5, EDNRB, LAPTM, FOXB, OTX2, NRIF2, SOX1 and HBE.