DEGs in the skin and brain
Through KEGG enrichment analysis of differentially expressed genes in
skin (Fig 5 A), 7 skin feeding related pathways were screened:
Arachidonic acid metabolism pathway, Adhesion pathway, Apoptosis,
Steroid biosynthesis, Tuberculosis, P53 signaling pathway, serotonergic
synapse. The genes in the pathway were analyzed and ten significantly
different genes associated with lactation were selected for validation.
These DEGs included JUNB, MRC, DPP3, CASP3, PPID, ITGA11, ALOXE3, HBE,
PTPRJ, GALE. Identically, through KEGG enrichment analysis of
differentially expressed genes in brain (Fig 5 B), 20 lactation related
pathways were screened: Estrogen signaling pathways, Inflammatory
medlator regulation of TRP channels, non-small cell lung cancer,
Vascular smooth muscle contraction, GnRH signaling pathway ,
TypeⅡdiabetes mellitus, Neurotrophic signaling pathway, NOD-like
receptor signaling pathway, Taste transduction, circadian rhythm,
Jak-STAT signaling pathway, insulin resistance, Fc gamma R-mediated
phagocytosis, Serotonergic synapses, Autophagy-animal, Small cell lung
cancer, Pathways in cancer, AGE-RAGE signaling pathway in diabetic
complications, Cytokin-cytokine receptor interaction. These DEGs
included PRKCD, H1-5,
EDNRB,
LAPTM, FOXB, OTX2, NRIF2, SOX1 and HBE.