Experimental fish
The breeding fish of discus is provided by the aquarium lab of Shanghai
Ocean University. Six pairs of breeding discus were taken from the same
litter of two breeding discus. These 6 pairs of breeding fish were
raised in 6 glass tanks of the same size. The tank size was 40×40×40cm,
and the effective water body was 40×40×30cm. All aquatics tanks are kept
continuously aerated for 24 hours, and the temperature can be adjusted
by the air in the aquatics workshop. During the experiment, the
dissolved oxygen in the water in the aquaculture tank was kept above 5
mg/L and was close to saturation. The water temperature was 28.5 ± 0.3℃
and pH 6.1 ± 0.2. During the experimental culture, the light was from
8:00 to 20:00 every day to ensure the illumination for 12 hours every
day. Feeding frozen blood worms
twice a day to the breeding fish accounts for 6%~7% of
the body weight of the breeding fish. Feeding time is 8:00 and 18:00
respectively. Clean the aquiculture tank every day and replace 1/4
volume of the effective water body. Wait for the breeding fish natural
reproduction.