Quantitation of PGE2 and estradiol by ELISA
PGE2 concentrations in sera were determined using
Prostaglandin E2 Express ELISA Kit (Cayman Chemical, Ann
Arbor, MI, USA) in accordance with the previous report [16].
Estradiol concentrations in sera were determined using Estradiol ELISA
Kit (Cayman Chemical) following the manufacturer’s instructions.