Physical observations
The body weights of the mice in each group (NC, XC, and PP242-treated) were measured once in every two days and compared (Fig. 1). Compared to the NC group, the body weights of the XC group mice decreased, but the changes were not significant. In contrast, the body weights of the PP242-treated mice decreased significantly and quickly in the first couple of days after treatment compared to the NC group, and later, the body weights gradually recovered. However, no significant change in body weight was observed between the XC and PP242-treated groups, indicating that the change in tumor size and drug treatment did not seriously affect body weight within 21 days.
Tumor-bearing mice were treated with vehicle and/or PP242 over the course of 21 days, and the size of the tumor in the right flank region was measured at two day intervals using digital caliper in the LS174T xenograft groups (XC and PP242-treated) in order to evaluate the drug therapeutic effects in vivo . After two weeks of PP242 treatment, a very significant difference in the tumor volumes was observed (p<0.001) before the mice were sacrificed (Fig. 2A and 2B). The tumors were separated after sacrifice and weighed, which further displayed the significant decrease in tumor weight for the PP242 treatment compared to the XC group mice (Fig. 2C).