Cell lines
The human colon cancer cell line LS174T was purchased from American Type
Culture Collection (ATCC; Manassas, VA, USA) and maintained in
Dulbecco’s modified eagle’s medium (DMEM) supplemented with 10% fetal
bovine serum (FBS) and 1% penicillin-streptomycin in a humidified 5%
CO2 and 37 °C incubator.