Serum biochemical parameters and histopathological tests
The results of the serum biochemical parameters test are shown in Fig.
3. All the measured serum biochemical parameters related to liver
toxicity were in the normal range for all groups. No significant
abnormalities were observed in the kidney toxicity biomarkers CRE and
BUN either. BUN was significantly reduced only in the PP242-treated
group compared to the NC group, but all were in the normal range.
Alterations in cholesterol levels were observed, where LDL levels were
reduced and HDL levels were increased after three weeks of PP242
treatment.
Histological examination of the liver showed no abnormal findings in all
subjects tested in the NC, XC and PP242-treated groups (Supplementary
Table 1, Supplementary Fig. 1). In the histologic examination of the
kidneys, no abnormal lesions were observed in any group except for
cortical renal tubule hypertrophy that was observed in one-fifth of the
PP242-treated group (Supplementary Table 1). However, it was thought
that there was no kidney toxicity since the degree of hypertrophy was
very minimal and the serum biochemical parameters for kidney function
were normal (Supplementary Fig. 1).
Taken together, no unusual toxicological changes were observed in the
liver or kidney when PP242 was administered according to the given
experimental design.