Cladribine (0.1-1 µM) decreases the motility of LPS-stimulated microglia
Microglia motility after CdA treatment was measured by random migration: track displacement refers to the bee-line distance the cells migrate in 24 hours, while the total distance travelled is the actual distance covered by the cells. Microglia were stained with cell tracker and treated with DMSO, LPS, DMSO and LPS, different concentrations of CdA, or CdA together with LPS. Movies were recorded, and the track displacement and total distance travelled by the microglia were determined.
The microglia track displacement was significantly increased by LPS stimulation compared to untreated control (Figure 3B ). Treatment with CdA 0.1 µM significantly decreased the track displacement of LPS-stimulated microglia, whereas CdA (0.1-10 µM) did not alter the track displacement of naïve microglia compared to DMSO control (Figure 3B ).
The total distance travelled by microglia was not significantly increased by LPS stimulation, though a trend was observed (p=0.07) (Figure 3C ). CdA in the range of 0.1-1 µM significantly decreased the total distance travelled by LPS-stimulated microglia (Figure 3C ). CdA 0.1-10 µM did not have an effect on the total distance travelled by naïve microglia compared to DMSO control (Figure 3C ).