Microglia cell culture
Mixed primary glial cell cultures were prepared by enzymatic
dissociation of postnatal P0-P5 mouse brains using Neural Tissue
Dissociation Kit (P) (Miltenyi Biotec, 130-092-628). The cells were
allowed to proliferate for 10 days prior to isolation of microglia by
the “shake off” method (19). The isolated microglia were on average
95% pure, when characterized as
CD11b+/CD45low on the flow cytometer
(BDTM LSR II, BD biosciences).