Figure 2
The effect of cladribine
on microglia granularity and size
Microglia were treated with DMSO
(28.2 mM), LPS (10 ng/ml), DMSO and LPS, different concentrations of CdA
(0.01-10 µM), and different concentrations of CdA together with LPS for
24 hours. A. A representative example of size (FSC) and
granularity (SSC) of microglia by flow cytometry. B. The effect
of CdA on the granularity (SSC) of naïve and LPS-stimulated microglia
relative to DMSO control. C. The effect of CdA on the size
(FSC) of naïve and LPS-stimulated microglia relative to DMSO control.
***p≤0.001, n=3-4 in each group, one-way ANOVA followed by multiple
comparisons tests, mean±SD.