RNA extraction and quantitative PCR
RNA was extracted using RNeasy micro kit (Qiagen, Denmark) and reverse
transcribed using High Capacity cDNA Reverse Transcription kit (Applied
Biosystems, 4374966). Quantitative PCR (qPCR) was performed on Bio-Rad
CFX ConnectTM Real-Time System (Software Bio-Rad CFX
Manager 3.1) using SYBR green chemistry (Thermo Scientific) and
corresponding primers (Supplementary Table 1 ). The expression
levels are reported relative to the geometric mean of
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and hypoxanthine
phosphoribosyltransferase 1 (HPRT1).