The effect of cladribine on the mRNA expression of TNF receptors in IL-4-stimulated microglia
mRNA expression of TNFR1 and TNFR2 was quantified by qPCR after microglia were treated with DMSO (28.2 mM), IL-4 (20 ng/ml), DMSO and IL-4, and different concentrations of CdA (0.01-10 µM) together with IL-4 for 24 hours. The expression is shown relative to DMSO control.
n=3-4 in each group, one-way ANOVA followed by multiple comparisons tests, mean±SD.