The effect of cladribine on the mRNA expression and protein secretion of cytokines by LPS-stimulated microglia
A. mRNA expression of IL-1β, TNF and IL-10 was quantified by qPCR after microglia were treated with DMSO (28.2 mM), LPS (10 ng/ml), DMSO and LPS, and different concentrations of CdA (0.01-10 µM) together with LPS for 24 hours. The expression is shown relative to DMSO control.B. The protein concentration of IL-1β, TNF and IL-10 in the microglia cell culture supernatant was quantified by Meso Scale. The microglia were treated with DMSO (28.2 mM), DMSO and LPS, and different concentrations of CdA (0.1-10 µM) together with LPS for 24 hours.
*p≤0.05, **p≤0.01, ***p≤ 0.001, ****p≤0.0001, n=4 in each group, unpaired t test with Welch’s correction and one-way ANOVA followed by Dunnett’s multiple comparisons test, mean±SD.