Cladribine (0.1-1 µM) decreases the phagocytotic ability of LPS-stimulated microglia
To investigate the effect of CdA on phagocytosis by naïve and LPS-stimulated microglia, the cells were incubated with fluorescent latex beads, and analyzed by flow cytometry. LPS stimulation significantly increased phagocytosis compared to control, while treatment with cytochalasin D, an inhibitor of actin polymerization, reduced phagocytosis (Figure 3A ). CdA in the range of 0.1-1 µM significantly decreased phagocytosis by LPS-stimulated microglia, while treatment with CdA (0.01-10 µM) did not affect phagocytosis by naïve microglia compared to DMSO control (Figure 3A ).