Figure legends
Fig. 1 Positivity rate comparison
between metagenomic next-generation sequencing (mNGS) and microbial
culture for bacterial infections (n = 14), fungal infections (n = 21),
general infectious (n = 43). The number of positive samples (y-axis) for
mNGS is plotted against the mNGS and microbial culture for bacterial
infections (n = 14), fungal infections (n = 21), general infectious
groups (x-axis).
Fig.2 Positivity rate comparison between metagenomic next-generation
sequencing (mNGS) of transbronchial lung biopsy (TBLB), bronchoalveolar
lavage fluid (BALF) and bronchial needle brushing (BB) samples, and mNGS
of TBLB + BALF + BB for bacterial
infections (n = 14), fungal infections (n = 21), general infectious (n =
43). The number of positive samples (y-axis) for mNGS is plotted against
the TBLB, BALF, BB and TBLB + BALF + BB groups (x-axis).
Fig. 3 A: typical septate hyphae with sharp-angled bifurcation could be
found with rapid on-site cytological evaluation (ROSE), the red arrow
refers to sharp-angled bifurcation, and the green arrow refers to the
septate of the hyphae. (Diff-Quik [DQ] stain, 1000 × magnification);
B ~ D: Cryptococcus was detected by ROSE in three
patients, the blue arrow refers to Cryptococcus (DQ stain, 1000 ×
magnification).
Fig. 4 Comparison of relative abundance of bacterial pathogens, fungal
pathogens and viral pathogens in patients with bacterial (A), fungal (B)
and viral (C) infections, respectively against the three types of
specimens (transbronchial lung biopsy (TBLB), bronchoalveolar lavage
fluid (BALF) and bronchial needle brushing (BB) specimens).
Fig. 5 Comparison of relative abundance of Prevotella (A), Neisseria
(B), Veillonella (C), Streptococcus (D), Fusobacterium (e) and Rothia
(F) in all of the researched objects against the three types of
specimens (transbronchial lung biopsy (TBLB), bronchoalveolar lavage
fluid (BALF) and bronchial needle brushing (BB) specimens).
Fig. 6 common oropharyngeal microbiota richness in TBLB, BALF and BB
specimens.