Figure 1 The BrVIN3.1-interacting protein BrHIS4.A04 promotes flowering in Arabidopsis.
(A) One-to-one yeast two hybrid assay of the interaction between AD-BrHIS4.A04 and BD-BrVIN3.1. (B) The interaction between AD-BrVIN3.1 and BD-BrHIS4.A04 was confirmed by X-gal staining. (C, D) Expression analysis of BrHIS4.A04 during different growth stages (C) and in different tissues of Chinese cabbage (D) Transcripts ofBrHIS4.A04 can be detected in all tissues or organs tested, including roots (r), stems (st), leaves (l), inflorescences (in), flowers (f) and 10-day-old seedlings (s), and it increased gradually before flowering and decreased after flowering. (E) Six-week-oldBrHIS4.A04OE plants flower earlier than the Col-0 controls under long day (16 h/8 h day/night, LD) conditions. (F) Numbers of rosette leaves on Col-0 andBrHIS4.A04OE plants at bolting under LD conditions; BrHIS4.A04OE plants bolted earlier than Col-0. (G to H) Real-time (RT)-PCR analysis of FLC andFT expression in 5-week-old Col-0 andBrHIS4.A04OE plants before flowering;FLC expression was reduced and FT expression was increased in BrHIS4.A04OE plants. Error bars indicate ± standard error (SE) of the mean. Columns labeled with different letters are statistically different according to the Student’s t-test (P <0.05).