Figure 5 Overexpressing BrHIS4.A04 leads to increased
chromatin H4 acetylation in Arabidopsis plants.
(A) H4 acetylation levels in five-week-old Col-0 andBrHIS4.A04OE plants with or without drought
were determined by Western blotting using anti-H4 (acetyl K5+K8+K12+K16)
antibody. The H4 protein level was used as an internal control. Under
normal growth conditions, a higher level of H4 acetylation was observed
in BrHIS4.A04OE plants. After drought
treatment, the BrHIS4.A04 acetylation level increased dramatically in
Col-0 plants; however, no increase was found inBrHIS4.A04OE plants. (B-F) The BrHIS4.A04
acetylation level at the ABI1, MYC2, FT , and FLC loci was
determined by ChIP followed by RT-PCR analysis. Under normal growth
conditions, the levels of H4 acetylation at all the tested loci were
much higher in BrHIS4.A04OE plants compared to
Col-0 plants. After drought treatment, the H4 acetylation levels of
these genes increased dramatically in Col-0, but remained at about the
same level as in theBrHIS4.A04O E plants grown
under normal conditions. Error bars indicate ± standard error (SE) of
the mean. Columns labeled with different letters are statistically
different according to the Student’s t-test (P <0.05).