Figure 2 Overexpression of BrHIS4.A04 confers drought hypersensitivity
(A) BrHIS4.A04OE plants are more severely affected than Col-0 plants after 3 weeks of drought treatment. (B) Water content and (C) ion leakage rate in 6-week-old Col-0 andBrHIS4.A04OE plants with or without drought treatment. There were no differences in the water contents and ion leakage rates between the BrHIS4.A04OE and Col-0 plants grown under normal conditions; however, after drought stress, the water contents of the BrHIS4.A04OEplants were reduced to ~70% of the control (Col-0), while the ion leakage rates increased between 5- and 6-fold compared to the control plants. (D-G) RT-PCR analysis of drought and ROS responsive genes. The relative degree of induction in the drought (D) and ROS (E to G) response genes was less in BrHIS4.A04OEplants compared to Col-0. Error bars indicate ± standard error (SE) of the mean. Columns labeled with different letters are statistically different according to the Student’s t-test (P <0.05).