Figure 3 BrHIS4.A04 leads to drought hypersensitivity dependently of ABA
Images of stomata from 6-week-old Col-0 andBrHIS4.A04OE plants grown under normal conditions (A to C) or after 3 weeks of drought treatment (D to F). Scale bars represent 50 μm. (G) Stomatal density, (H) guard cell length, and (I) stomatal aperture size were measured in 6-week-old Col-0 andBrHIS4.A04OE plants with or without drought treatment. BrHIS4.A04OE plants had larger stomatal apertures under drought conditions. RT-PCR analysis of ABA synthesis (J, K) and ABA signaling (L, M) genes showed that the expression of the four ABA genes was higher inBrHIS4.A04OE plants compared with Col-0 plants grown under normal conditions, and was induced in both plants after drought treatment. However, the induction was to a much lesser degree inBrHIS4.A04OE plants. Error bars indicate ± standard error (SE) of the mean. Columns labeled with different letters are statistically different according to the Student’s t-test (P <0.05).