Figure 4 BrHIS4.A04 prevents premature flowering under drought
conditions.
(A) Flowering in 6-week-old Col-0 andBrHIS4.A04OE plants grown under normal and
drought conditions. For drought treatment, 3-week-old Arabidopsis
seedlings were deprived of water for three weeks. (B) Rosette leaf
numbers of Col-0 and BrHIS4.A04OE plants at
bolting. The drought-treatedBrHIS4.A04O E plants bolted at
roughly the same time as the normal-grown plants. (C to F) RT-PCR
analysis of flowering genes in 5-week-old Col-0 andBrHIS4.A04OE plants. Under normal growth
conditions, the expression of BrHIS4.A04OEresulted in increased levels of GI -, FT , andSOC1 -specific mRNA (D, E ), and slightly reduced the mRNA
level of FLC ; after drought treatment, a slight reduction inFLC and increases in GI, FT , and SOC1 expression
were found in Col-0 plants (C-F ). However, there was no
increase in the expression of GI, FT , and SOC1 inBrHIS4.A04OE plants. Error bars indicate ±
standard error (SE) of the mean. Columns labeled with different letters
are statistically different according to the Student’s t-test (P
<0.05).