Figure 1 The BrVIN3.1-interacting protein BrHIS4.A04 promotes
flowering in Arabidopsis.
(A) One-to-one yeast two hybrid assay of the interaction between
AD-BrHIS4.A04 and BD-BrVIN3.1. (B) The interaction between AD-BrVIN3.1
and BD-BrHIS4.A04 was confirmed by X-gal staining. (C, D) Expression
analysis of BrHIS4.A04 during different growth stages (C) and in
different tissues of Chinese cabbage (D) Transcripts ofBrHIS4.A04 can be detected in all tissues or organs tested,
including roots (r), stems (st), leaves (l), inflorescences (in),
flowers (f) and 10-day-old seedlings (s), and it increased gradually
before flowering and decreased after flowering. (E) Six-week-oldBrHIS4.A04OE plants flower earlier than the
Col-0 controls under long day (16 h/8 h day/night, LD) conditions. (F)
Numbers of rosette leaves on Col-0 andBrHIS4.A04OE plants at bolting under LD
conditions; BrHIS4.A04OE plants bolted earlier
than Col-0. (G to H) Real-time (RT)-PCR analysis of FLC andFT expression in 5-week-old Col-0 andBrHIS4.A04OE plants before flowering;FLC expression was reduced and FT expression was increased
in BrHIS4.A04OE plants. Error bars indicate ± standard
error (SE) of the mean. Columns labeled with different letters are
statistically different according to the Student’s t-test (P
<0.05).