Fungal communities
e
[CHART]
Bacterial communities
[CHART]
Fungal communities
f
Figure 3. Gut bacterial community variation due to lifestyle changes. (a) Variation in gut microbiome richness during lifestyle shifts. Circles represent samples, with Wild-training I panda communities shown in blue, wild-training II in red, reintroduced in green, and wild in purple. (b) Variation in gut microbiome diversity during lifestyle shifts. Circles represent samples, as indicated in panel a. (c) Principal Coordinates Analysis (PCoA) of bacterial community structures from the wild-training I, wild-training II, reintroduced, and wild lifestyle groups. PC1 and PC2 are shown on the x and y axes along with the percent variation explained by each. Circles represent samples and are colored as indicated in panel a. (d) Differences in overall bacterial phylum-level compositions of the communities from the wild-training I, wild-training II, and reintroduced pandas. (e) Significantly different bacterial taxa among the wild-training I, wild-training II, and reintroduced groups, as identified by linear discriminant analysis coupled with effect size (LEfSe) using the default parameters. Blue symbols show wild-training I samples, red: wild-training II, and green: reintroduced. Blue text shows phyla and genera. (f) Differences in overall bacterial genus-level composition of the communities from the wild-training I, wild-training II, and reintroduced experimental groups. *: 0.01 < p< 0.05, **: p <0.01.