2.4.2 Larvae stimulated by fresh ripe fuji apples or crown pears
Fourth instar larvae were obtained that had been starved for 1 day. For each experiment, fresh ripe fuji apples (Yantai, Shandong, China) or crown pears (Baoding, Hebei, China) were put in the corners of two different plexiglas cages (50 × 50 × 50 cm) , and 60 starved larvae were released in the center of plexiglas cages and isolated with a container to avoid feeding (Figure S1 B). After 2 h, the head of odor stimulated and unstimulated larvae were collected and quickly frozen in the liquid nitrogen. Expression levels of GmolCXEs in head of odor stimulated and unstimulated larvae were measured by qPCR. Three biological replicates were performed.