2.2.1 Sequence analysis
Candidate GmolCXEs were identified through keyword screening of the BLASTX annotations of transcriptomic data from G. molestalarva heads (fourth instar larvae, fifth instar larvae for 1-2 days and fifth instar larvae for 6-7 days). The open reading frames (ORFs) of all genes were predicted using the NCBI ORF finder. Similarity searches were performed with the NCBI-BLAST network server (http://blast.ncbi.nlm.nih.gov/). Putative N-terminal signal peptides were predicted using the SignalP 5.0 server (http://www.cbs.dtu.dk/services/SignalP/ ). The theoretical isoelectric points and molecular weights of the deduced proteins were calculated using the ExPASy tool (http://web.expasy.org/compute_pi/ ). Catalytic residues were predicted by searching the NCBI Conserved Domain Database (http://www.ncbi.nlm.nih.gov/structure/cdd/cdd.shtml).