3.1 Identification and phylogenetic analysis of candidateGmolCXEs
Based on the bioinformatic analysis, 23 candidate GmolCXEs were
identified in the larval head transcriptome of G. molesta . These
sequences were named GmolCXE1-23 according to the similarity to
known Lepidopteran CXEs and numbers of amino acids in open reading
frames (ORFs) (Table 1). Among them, 15 GmolCXEs contained
full-length ORFs, and the rest 8 sequences were partial. The 15
full-length GmolCXEs contain a conserved motif pattern including
an oxyanion hole (GGA, etc.), a catalytic triad (S-Xa-E-Xb-H), and a
conserved Ser active site (G-X-S-X-G) (Figure S2 and Table 2). The
predicted theoretical isoelectric points of these esteases are ranged
from 5.22 to 9.27, and calculated molecular masses varies from 58.79 kDa
to 74.82 kDa (Table 2). Phylogenetic analysis showed that 23 putativeGmolCXEs are closely clustered into 7 different clades (Figure
1). These clades include moth antennal esterases (GmolCXE1 ,2 , 14 , 15 , 17 , 18 , 20 ),
cuticular and antennal esterases (GmolCXE5 ), ß-esterases and
pheromone esterases (GmolCXE13 , 21 ), lepidopteran juvenile
hormone esterases (JHE) (GmolCXE7 ), mitochondrial and cytosolic
esterases (GmolCXE3a , 3b , 4 , 6 , 10 ,12 , 16 ), α-esterases (GmolCXE8 , 9 ,11 , 19 ) and neuroligins (GmolCXE22 ).GmolCXE1 and GmolCXE13 are closely clustered withApolODE and ApolPDE of Antheraea Polyphemus ,
respectively (Figure 1). All sequence information of these 23GmolCXEs has been released in the GenBank database (Table 1).