2.4.2 Larvae stimulated by fresh ripe fuji apples or crown
pears
Fourth instar larvae were obtained that had been starved for 1 day. For
each experiment, fresh ripe fuji apples (Yantai, Shandong, China) or
crown pears (Baoding, Hebei, China) were put in the corners of two
different plexiglas cages (50 × 50 × 50 cm) , and 60 starved larvae were
released in the center of plexiglas cages and isolated with a container
to avoid feeding (Figure S1 B). After 2 h, the head of odor stimulated
and unstimulated larvae were collected and quickly frozen in the liquid
nitrogen. Expression levels of GmolCXEs in head of odor
stimulated and unstimulated larvae were measured by qPCR. Three
biological replicates were performed.