Male adults were disturbed in the search for female adults by GmolCXEs silencing
After RNAi for 24 h, the expression levelsĀ of GmolCXE1 and GmolCXE5 decreased significantly (Figure 4). The expression ofGmolCXE1 and GmolCXE5 decreased by 56.46% and 47.62%, respectively (Figures 4 A and B). The number of male adults choosing female adults had a significant reduction by GmolCXE1 silencing, while no difference was observed by GmolCXE5 silencing (P> 0.05) (Figure 4 C). The male adults injected withdsCXE1 and dsCXE5 spent much time and exhibited more frequencies of turning back during the searching period in the Y-tube compared to male adults injected with dsEGFP as control (Figure 4 D and E). EAG amplitudes in response to sex pheromone mix components (Z8-12: Ac: E8-12: Ac = 9: 1) released by female moths were significantly reduced when GmolCXE1 orGmolCXE5 were knocked down in male adults by RNAi (Figure 4 F).