3.2 Screening of five target GmolCXEs involved in odor reception of foraging and mating
In male and female antennae of G. molesta adults, the relative expression levels of GmolCXE1 and GmolCXE5 were significantly higher than other esterase genes (Figure 2 A). TheGmolCXE1 and GmolCXE5 were widely expressed in all tissues of adults in both male and females, whereas their expression in antennae were higher than other tissues (Figure 2 B and C). After female adult stimulation, the expressions of GmolCXE1 andGmolCXE5 in the male antennae was induced by about 2- folds (Figure 2 D).
In the 4th instar G. molesta larvae, the expressions of seven esterase genes (GmolCXE7 , 10 ,13 , 14 , 20-22 ) were significantly higher in the head than in the abdomen (Figure 3 A). Among these genes, the expressions of GmolCXE13 , GmolCXE14 and GmolCXE21in the larva head were significantly up-regulated as much as twice after fresh ripe fuji apples and crown pears stimulations than those in the control (Figure 3 B).