2.2.2 Phylogenetic tree construction
The amino acid sequences of GmolCXEs and CXEs from other
species were aligned using ClustalX 2.0 (Larkin et al., 2007). A
neighbor-joining tree was constructed by the program MEGA 8.0 using the
neighbor-joining method with a p-distance model and a pairwise deletion
of gaps (Tamura et al., 2013; Tan et al., 2018). Node support was
assessed using a bootstrapping procedure with 1,000 replicates. And the
Fig tree 4.3 is used as the auxiliary software to modify the tree (Wei
et al., 2017). The protein names and accession numbers corresponding to
the genes used for construction of the phylogenetic tree are listed in
Table S1.