GmolCXEs degraded free ester volatiles in foraging
After purified by Ni ion affinity chromatography (Figure S3), proteins
of GmolCXE14 and GmolCXE21 were incubated with ethyl butanoate or ethyl
hexanoate for 1 h and the products were submitted to GC-MS analysis.
GC-MS results showed peak areas of two ester compounds treated with
purified GmolCXE14 and GmolCXE21 were significantly reduced compared to
the control groups (Figure 8 D-I). The degradation percentage of ethyl
butanoate were 94.03% (CXE14) and 87.23% (CXE21) (Figure 8 J), and
degradation percentage of ethyl hexanoate were 50.32% (CXE14) and
16.88% (CXE21) (Figure 8 K).