2.2.1 Sequence analysis
Candidate GmolCXEs were identified through keyword screening of
the BLASTX annotations of transcriptomic data from G. molestalarva heads (fourth instar larvae, fifth instar larvae for 1-2 days and
fifth instar larvae for 6-7 days). The open reading frames (ORFs) of all
genes were predicted using the NCBI ORF finder. Similarity searches were
performed with the NCBI-BLAST network server
(http://blast.ncbi.nlm.nih.gov/).
Putative N-terminal signal peptides were predicted using the SignalP 5.0
server (http://www.cbs.dtu.dk/services/SignalP/ ). The theoretical
isoelectric points and molecular weights of the deduced proteins were
calculated using the ExPASy tool
(http://web.expasy.org/compute_pi/ ). Catalytic residues were
predicted by searching the NCBI Conserved Domain Database
(http://www.ncbi.nlm.nih.gov/structure/cdd/cdd.shtml).