2.3.1 Tissue collection for gene expression
The head and abdomen of 15 fourth instar larvae were collected for three biological replicates. The antennae, head, thorax, abdomen, legs and wings for both sexes at 3 days after adult eclosion were collected and kept in different microcentrifuge tubes (1.5 mL, no RNAase). Three biological replicates (100 individuals per replicate) were performed for adult samples. All samples were frozen and stored in liquid nitrogen until the RNA extraction.