2.3.1 Tissue collection for gene expression
The head and abdomen of 15 fourth instar larvae were collected for three
biological replicates. The antennae, head, thorax, abdomen, legs and
wings for both sexes at 3 days after adult eclosion were collected and
kept in different microcentrifuge tubes (1.5 mL, no RNAase). Three
biological replicates (100 individuals per replicate) were performed for
adult samples. All samples were frozen and stored in liquid nitrogen
until the RNA extraction.