Figure 5 : Copy number measured on 35 parafilm slips
spiked with brown-marmorated stinkbug fecal slurry and kept indoors, in
darkness, for one of seven time intervals (from 0-144 hours with 5
replicates per time interval). Samples were suspended in water and
separated into intracellular and extracellular DNA via serial filtration
(10 and 0.2 µm filters, respectively). The curves represent Poisson
Generalized Linear Models (GLM; log link) with non-parametric
bootstrapped 95% confidence intervals. The 95% confidence intervals
for the slope parameters of these lines overlapped zero (intracellular:
0.00647 [-0.00949, 0.02243]; extracellular: 0.00562 [-0.00025,
0.01150]) indicating a lack of degradation over the course of the
experiment. Decay curves were not fit with non-linear least squares as
with the outdoor experiments due to problems achieving model
convergence. The upper and lower dashed lines below the points indicate
detection thresholds representing 39 and 40 PCR cycles, respectively.