Persistence under simulated rainfall
We found 220 ml of simulated rainfall, irrespective of whether it was
heavy, light, or a mist, produced no positive eDNA detections across all
eight qPCR replicates (Table 1 ). Reducing the volume of mist to
110 ml resulted in a single positive qPCR replicate on tomatoes, while
peaches produced four out of eight positive qPCR replicates over four
sample replicates (Table 1 ). We found 55 ml on tomatoes
produced two qPCR positives over a single sample replicate, and peaches
produced three qPCR positives over two sample replicates. We found the
results for tomatoes were unchanged with 27 ml of water, whereas peaches
produced one qPCR positive (Table 1 ).