Figure legends
Figure 1. Principal component analyses of the offspring groups using
normalized counts (i.e., log-CPM) of the 8,880 genes retained post
filtering. The maternal parent is listed prior to the paternal parent
for the abbreviation of the offspring groups, where “T” is A.
tenuis and “L” is A. loripes .
Figure 2. (a ) The number of up or down regulated genes between
the pairs of offspring groups (padj < 0.05 and
log-fold change > 0.2). (b ) Venn diagram showing the
number of differentially expressed genes (DEGs) between the pairs of
offspring groups. The overlapping space between the circles indicates
the number DEGs in both pairs of comparison. The abbreviation of the
offspring groups is that the first letter indicates maternal parent and
the second letter the paternal parent, where “T” is A. tenuisand “L” is A. loripes .
Figure 3. Heatmap of the 500 most variable genes across samples based on
a calculated matrix of Euclidean distances from the log-CPM. “T”
refers to A. tenuis and “L” refers to A. loripes in the
offspring group abbreviation, and the maternal parent is listed prior to
the paternal parent.
Figure 4. Volcano plots showing the log-fold change and p-values
(padj) of the 8880 genes retained post filtering. Dotted
lines indicate the p-value cut off (< 0.05) and log-fold
change (LFC) cut off (> 5). Note that the LFC cut off
applied throughout this study was 0.2, and the LFC cut off
(> 5) applied here was only to visualize the genes that
were most highly differentially expressed. Genes that pass both p-value
and a LFC cut off of > 5 are in red. Gene names of shared
genes between the two pairs of comparison are highlighted in blue.
(a ) Paternal purebred LL compared to its hybrid TL; (b )
Hybrid LT compared to hybrid TL.