Figure legends
Figure 1. Principal component analyses of the offspring groups using normalized counts (i.e., log-CPM) of the 8,880 genes retained post filtering. The maternal parent is listed prior to the paternal parent for the abbreviation of the offspring groups, where “T” is A. tenuis and “L” is A. loripes .
Figure 2. (a ) The number of up or down regulated genes between the pairs of offspring groups (padj < 0.05 and log-fold change > 0.2). (b ) Venn diagram showing the number of differentially expressed genes (DEGs) between the pairs of offspring groups. The overlapping space between the circles indicates the number DEGs in both pairs of comparison. The abbreviation of the offspring groups is that the first letter indicates maternal parent and the second letter the paternal parent, where “T” is A. tenuisand “L” is A. loripes .
Figure 3. Heatmap of the 500 most variable genes across samples based on a calculated matrix of Euclidean distances from the log-CPM. “T” refers to A. tenuis and “L” refers to A. loripes in the offspring group abbreviation, and the maternal parent is listed prior to the paternal parent.
Figure 4. Volcano plots showing the log-fold change and p-values (padj) of the 8880 genes retained post filtering. Dotted lines indicate the p-value cut off (< 0.05) and log-fold change (LFC) cut off (> 5). Note that the LFC cut off applied throughout this study was 0.2, and the LFC cut off (> 5) applied here was only to visualize the genes that were most highly differentially expressed. Genes that pass both p-value and a LFC cut off of > 5 are in red. Gene names of shared genes between the two pairs of comparison are highlighted in blue. (a ) Paternal purebred LL compared to its hybrid TL; (b ) Hybrid LT compared to hybrid TL.