2.1 Cultivation of S. peregrina
Adult specimens of S. peregrina were trapped with pork liver bait
in Changsha, Hunan Province, China, and raised at 25 ± 1°C and 70 ± 5%
relative humidity with a photoperiod regime of 12:12h light/darkness in
an artificial climate chamber, employing pork liver as a medium for
larvipositing and larval rearing.
In
order to reduce genetic variability, mating pairs of adult S.
peregrina trapped in the wild were highly inbred for six generations.
Afterwards, 3rd-instar larvae and newly hatched adult females were used
for further studies.