2.1 Cultivation of S. peregrina
Adult specimens of S. peregrina were trapped with pork liver bait in Changsha, Hunan Province, China, and raised at 25 ± 1°C and 70 ± 5% relative humidity with a photoperiod regime of 12:12h light/darkness in an artificial climate chamber, employing pork liver as a medium for larvipositing and larval rearing. In order to reduce genetic variability, mating pairs of adult S. peregrina trapped in the wild were highly inbred for six generations. Afterwards, 3rd-instar larvae and newly hatched adult females were used for further studies.