VRN and Rht gene segregation in DH populations
A screen of a suite of phenology genes in the DH lines identified 14
haplotypes segregating for VrnA1, VrnB1, VrnD1 , Rht1(Rht-B1 ), Rht2 (Rht-D1 ), and Ppd-B1 (Fig.
2c, Supplementary Fig. 2a-d). In DH populations, SpB and Spitfire x B7A
(Sp7A) segregated forVrnA1 , while VrnD1segregated in Spitfire/Mace (SpM) and BW, and VrnB1 in the BW,
SpB, Sp7A and St3B populations. For Rht1 and Rht2 , only
Suntop and Gregory carry both the Rht-B1b and the wild-typeRht-D1a alleles. The other six parental lines have the opposite
genotype for these alleles. Therefore, Rht1 and Rht2segregated in two populations of Suntop x B3B (St3B) and Gregory x B7A
(G7A). Copy number differences for the Ppd-B1 allele were
detected in parental lines and the segregations in SpB, Sp7A, G7A, and
St3B populations were expected (Supplementary Fig. 2d).