Infection of cotton seedlings and sample collection
Two-week-old seedlings of G. barbadense cv. 7124 and G.
hirsutum cv. YZ1 were infected with V. dahliae conidial
suspensions (1×10^6 conidia·ml-1)
using a root dip inoculation method, and control plants were
mock-inoculated with sterile water. Roots were harvested at 0 h, 6 h, 12
h, 24 h and 48 h post inoculation, frozen in liquid nitrogen and stored
at
-80°C
for subsequent RNA extraction.