Subcellular localization analysis
Agrobacterium tumefaciens strain GV3101 containing binary vectors with the GFP fusion protein were infiltrated into tobacco leaf epidermal cells for transient expression analysis, and the transfected cells were imaged by confocal laser scanning microscopy at 60 h after inoculation, according to Gao et al. (2016). The GFP was excited at a wavelength of 488 nm, and fluorescence emission was collected from 505 to 530 nm. Chlorophyll autofluorescence was excited at a wavelength of 488 nm and collected from 647 to 745 nm.