SUPPORTING INFORMATION
Table S1. Model of miR398 repression of the expression of NBS-LRR andCSD genes by inhibiting translation and mRNA cleavage, respectively. The predictions were accomplished using the plant small RNA target analysis server psRNATarget (http://plantgrn.noble.org/psRNATarget/). UPE: unpaired energy required to open secondary structure around miRNA’s target site on mRNA; Less energy represents higher possibility to be an effective target site.
Table S2. Primers used in this study. The primers used for reverse transcription-quantitative PCR (RT-qPCR) and qPCR are marked by ‘RL’; the primers for gateway vector construction are marked by ’attb’.
Figure S1: Southern blot and transgene expression analysis in cotton.
A: Southern blot analysis of transgenic plants. The single band from each lane represents the single T-DNA insertion of each transgenic line.
B and C: Reverse transcription-quantitative PCR analysis ofGhCSD2 and miR398b expression levels in miR398b-resistantGhCSD2 overexpressing (OEGhrCSD2) transgenic plants and miR398b-overexpressing transgenic plants, respectively. Values represent the means ± s. d. from three biological replicates.
Figure S2: Comparison of amino acid sequences of nucleotide binding site regions 4NB and 5NB in cotton proteins Ghir_A04G004640