Subcellular localization analysis
Agrobacterium tumefaciens strain GV3101 containing binary vectors
with the GFP fusion protein were infiltrated into tobacco leaf epidermal
cells for transient expression analysis, and the transfected cells were
imaged by confocal laser scanning microscopy at 60 h after inoculation,
according to Gao et al. (2016). The GFP was excited
at a wavelength of 488 nm, and
fluorescence emission was collected from 505 to 530 nm. Chlorophyll
autofluorescence was excited at a wavelength of 488 nm and collected
from 647 to 745 nm.