3.4 BiFC assay shows colocalization of PpINH1 andPpVIN2 in tobacco cells
PpINH1 is located at the cell membrane, in the cytoplasm, and in the nucleus, while PpVIN2 is located in the cell membrane and cytoplasm (Figure 3b ). When the genes are co-expressed in tobacco cells in a BiFC assay, PpINH1 and PpVIN2 co-localize (Figure 3c ). No appreciable fluorescence was observed when PpINH1 -YC was co-expressed with YN, when PpVIN2 -YN was co-expressed with YC, or when YC was co-expressed with YN. These results support the hypothesis that PpINH1 interacts with PpVIN2 in vivo .