3.2 Promoter analysis of PpINHs
VIN regulates sucrose metabolism in conjunction with INH interaction
proteins to enable plants to resist cold stress. To determine whetherPpINH gene expression is sensitive to low temperature,PpINH promoters (defined as regions 3000 bp in length directly
upstream from PpINH coding sequences) were examined in the peach
genome. CBF/DREB (C-repeat binding factor/dehydration responsive
element) binding sites occur within each PpINH promoter(Figure 2). The CBF/DREB protein binds specifically to G(TG)CGG
cis-elements involved in regulating plant responses to drought, low
temperature, and stress. These results support the hypothesis that INH
proteins act as key regulators in flowering plants to mediate the
response to cold stress, especially in dicots.