\documentclass[10pt]{article}

\usepackage{fullpage}
\usepackage{setspace}
\usepackage{parskip}
\usepackage{titlesec}
\usepackage[section]{placeins}
\usepackage{xcolor}
\usepackage{breakcites}
\usepackage{lineno}
\usepackage{hyphenat}





\PassOptionsToPackage{hyphens}{url}
\usepackage[colorlinks = true,
            linkcolor = blue,
            urlcolor  = blue,
            citecolor = blue,
            anchorcolor = blue]{hyperref}
\usepackage{etoolbox}
\makeatletter
\patchcmd\@combinedblfloats{\box\@outputbox}{\unvbox\@outputbox}{}{%
  \errmessage{\noexpand\@combinedblfloats could not be patched}%
}%
\makeatother


\usepackage{natbib}




\renewenvironment{abstract}
  {{\bfseries\noindent{\abstractname}\par\nobreak}\footnotesize}
  {\bigskip}

\titlespacing{\section}{0pt}{*3}{*1}
\titlespacing{\subsection}{0pt}{*2}{*0.5}
\titlespacing{\subsubsection}{0pt}{*1.5}{0pt}


\usepackage{authblk}


\usepackage{graphicx}
\usepackage[space]{grffile}
\usepackage{latexsym}
\usepackage{textcomp}
\usepackage{longtable}
\usepackage{tabulary}
\usepackage{booktabs,array,multirow}
\usepackage{amsfonts,amsmath,amssymb}
\providecommand\citet{\cite}
\providecommand\citep{\cite}
\providecommand\citealt{\cite}
% You can conditionalize code for latexml or normal latex using this.
\newif\iflatexml\latexmlfalse
\providecommand{\tightlist}{\setlength{\itemsep}{0pt}\setlength{\parskip}{0pt}}%

\AtBeginDocument{\DeclareGraphicsExtensions{.pdf,.PDF,.eps,.EPS,.png,.PNG,.tif,.TIF,.jpg,.JPG,.jpeg,.JPEG}}

\usepackage[utf8]{inputenc}
\usepackage[english]{babel}



\usepackage{float}








\begin{document}

\title{Genotypic differences in phosphate utilization efficiency (PUE) and
photosynthetic efficiency under P starvation and metabolic markers for
high PUE}



\author[1]{Mutsumi Watanabe}%
\author[2]{Yoshiaki Ueda}%
\author[2]{Katsuhiko Kondo}%
\author[3]{Satoru Ishikawa}%
\author[1]{Takayuki Toghe}%
\author[1]{Asdrubal Burgos}%
\author[1]{Yariv Brotman}%
\author[1]{Alisdair R. Fernie}%
\author[1]{Dirk Walther}%
\author[1]{Rainer Hoefgen}%
\author[2]{Matthias Wissuwa}%
\affil[1]{MPI of Molecular Plant Physiology}%
\affil[2]{Japan International Research Center for Agricultural Sciences (JIRCAS)}%
\affil[3]{National Agriculture and Food Research Organization (NARO)}%


\vspace{-1em}



  
  \date{May 08, 2024}


\begingroup
\let\center\flushleft
\let\endcenter\endflushleft
\maketitle
\endgroup





\selectlanguage{english}
\begin{abstract}
Utilizing phosphate more efficiently is crucial for sustainable crop
production. Highly efficient rice (Oryza sativa) cultivars have been
identified and this study aims to identify metabolic markers associated
with P utilization efficiency. P deficiency generally reduced leaf P
concentrations and CO2 assimilation rates but efficient cultivars were
reducing leaf P concentrations further than inefficient ones while
maintaining similar CO2 assimilation rates. Adaptive changes in carbon
metabolism were detected but equally in efficient and inefficient
cultivar groups. Groups furthermore did not differ with respect to
partial substitutions of phospholipids by sulfo- and galactolipids.
Metabolites significantly more abundant in the efficient group, such as
sinapate, benzoate and glucoronate, were related to antioxidant defense
and may help alleviating oxidative stress caused by P deficiency. Sugar
alcohols ribitol and threitol were another marker metabolite for higher
phosphate efficiency as were several amino acids, especially threonine.
Since these metabolites are not known to be associated with P
deficiency, they may provide novel clues for the selection of more P
efficient genotypes. In conclusion, metabolite signatures detected here
were not related to phosphate metabolism but rather helped P efficient
lines to keep vital processes functional under the adverse conditions of
P starvation.%
\end{abstract}%



\sloppy


\textbf{Hosted file}

\verb`P Rice ms_Feb24_notrack2003.doc`  available at \url{https://authorea.com/users/300561/articles/430281-genotypic-differences-in-phosphate-utilization-efficiency-pue-and-photosynthetic-efficiency-under-p-starvation-and-metabolic-markers-for-high-pue}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Fig1-wcap/Fig1-wcap}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Fig2-wcap/Fig2-wcap}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Fig3-wcap/Fig3-wcap}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Fig4/Fig4}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Fig5new/Fig5new}

\end{center}
\end{figure}\selectlanguage{english}
\begin{figure}[H]
\begin{center}
\includegraphics[width=0.70\columnwidth]{figures/Fig6/Fig6}

\end{center}
\end{figure}

\selectlanguage{english}
\FloatBarrier
\end{document}

