Chemicals and reagents
Foetal bovine serum (FBS, No. 10099141),
trypsin
(No.
27250018),
Dulbecco’s
modified Eagle’s medium (DMEM, No. THP5218), L-Glutamine (No. 1816801),
B-27 (No. 2004470), Neurobasal-A Medium (No. 1995169),
and
Roswell Park Memorial Institute 1640 (GI 1640) medium (No. 8119040) were
purchased from Gibco Invitrogen
Corporation
(Carlsbad,
CA, USA).
Collagenase I (No. C0130-1G) and collagen (type I solution from rat
tail, No. A10483-01)
were
obtained from Sigma-Aldrich (St. Louis, MO,
USA).
L-THP and OXA (No. D109812) were purchased from Aladdin Reagent Co.,
Ltd. (Shanghai, China).