CMS Inhibited Oxidative Stress and Inflammation of Retinal Cells
through Activating SIRT1
In order to evaluate whether CMS regulated oxidative stress and
inflammation in retinal cells with DR through SIRT1, we silenced SIRT1
expression in ARPE-19 cells. ELISA was performed to detect the
expression of oxidative stress-related factors, which observed that CMS
treatment obviously reduced the expression of ROS and MDA, but increased
SOD expression in ARPE-19 cells stimulated with HG-FFA, which could be
blocked by sh-SIRT1 (Table 3). In addition, the results of RT-qPCR and
western blot analysis demonstrated that iNOS expression in ARPE-19 cells
stimulated with HG-FFA was declined by CMS treatment, which could be
reversed by sh-SIRT1 (Figure 4A - B). Nitrite test revealed that CMS
treatment notably decreased the expression of NO in HG-FFA stimulated
ARPE-19 cells, which could be rescued by sh-SIRT1 (Figure 4C). According
to the result of ELISA, in ARPE-19 cells stimulated with HG-FFA, notably
decreased expression of IL-6, TNF-α, and CRP was observed after CMS
treatment, which could be reversed by sh-SIRT1 (Figure 4D - F).
Therefore, CMS was beneficial to alleviate the oxidative stress and
inflammation in retinal cells of DR through activating SIRT1.