CMS Inhibits Apoptosis of Retinal Cells of DR
At last, to measure the effect of CMS on apoptosis, we analyzed cell
apoptosis and examined the expression of apoptosis related factors in
ARPE-19 cells stimulated with HG-FAA. Flow cytometry suggested that the
apoptosis rate of ARPE-19 cells were notably decreased after CMS
treatment, which could be reversed by sh-SIRT1 (Figure 5A - B). Besides,
western blot analysis showed that ARPE-19 cells treated with CMS
presented lower expression of Cyt-C and Caspase-3, which could be
enhanced by sh-SIRT1 (Figure 5C). Taken together, all these data
suggested that CMS could inhibit apoptosis of retinal cells of DR
through activating the expression of SIRT1.