CMS Inhibits Apoptosis of Retinal Cells of DR
At last, to measure the effect of CMS on apoptosis, we analyzed cell apoptosis and examined the expression of apoptosis related factors in ARPE-19 cells stimulated with HG-FAA. Flow cytometry suggested that the apoptosis rate of ARPE-19 cells were notably decreased after CMS treatment, which could be reversed by sh-SIRT1 (Figure 5A - B). Besides, western blot analysis showed that ARPE-19 cells treated with CMS presented lower expression of Cyt-C and Caspase-3, which could be enhanced by sh-SIRT1 (Figure 5C). Taken together, all these data suggested that CMS could inhibit apoptosis of retinal cells of DR through activating the expression of SIRT1.