Enzyme Linked Immunosorbent Assay (ELISA)
The levels of IL-1β and TNF-α in the cell supernatant were detected by a ELISA kit (R & D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions with a smart spec-plus spectrophotometer (Bio Rad laboratories, Hercules, CA, USA) (Saferding, Puchner, 2017).