Transducer (“pathway”) |
β-arrestin 2 |
Gi
|
Gq
|
Cell line |
CHO |
CHO |
CHO |
Receptor expression levels (fmol/mg protein) |
300 |
300 |
300 |
Time point (min)
(For time-resolved, a span)
|
15-60
|
30
|
30
|
Temperature (°C) |
20 |
20 |
20 |
Reference ligand (endogenous) |
CP55,940 |
CP55,940 |
CP55,940 |
Reference ligand for Emax (if non-endogenous) |
- |
- |
- |
Measured process |
Recruitment |
Accumulation |
Accumulation |
Measured molecule 1 (a GPCR, transducer, effector or second
messenger) |
CB1
|
cAMP |
Myo-inositol-1-phosphate
(IP1) |
Measured molecule 2 (where applicable) |
β-arrestin 2 |
|
|
Signal detection technique |
FRET |
HTRF |
HTRF |