Cytokine detection
The serum was obtained by centrifugation at 1000 × g for 10 min and the content of IL-35 was detected by an AimPlex bead-based immunoassay (Quanto Bio, Beijing, China) according to the manufacturer’s instructions. All samples were analyzed by a FACS Calibur instrument and data were collected by Cell Quest Pro software (BD Biosciences, San Diego, CA, USA). Data were analyzed by FCAP Array V3.0 software (BD Biosciences).