Supporting Information
Additional Supporting Information may be found in the online version of
this article at the publisher’s web-site:
Table S1. Primers used in this study.
Table S2. The profiles of small RNA deep sequencing for
35S:SlmiR168a, 35S:rSlAGO1 and WT.
Table S3. List of all expressed miRNA in 35S:SlmiR-168a ,35S:rSlAGO1 and WT.
Table S4. The expressed conserved miRNAs were classified into
different miRNAs families.
Table S5. List of the differentially expressed miRNAs in35S:SlmiR-168a plants compared with WT.
Table S6. List of the differentially expressed miRNAs in35S:rSlAGO1 plants compared with WT.
Table S7. 334 miRNAs that were upregulated in the comparison of35S:rSlAGO1 and WT plants but downregulated in the comparison of35S:SlmiR-168a and WT plants ; 276 miRNAs that were downregulated
in the comparison of 35S:rSlAGO1 and WT plants but upregulated in
the comparison of 35S:SlmiR-168a and WT plants.
Table S8. List of the miRNAs whose target geFnes are predicted.
Table S9. Target predict annotation for the differentially
expressed miRNAs.
Table S10. miRNA/mRNA
pairs in the comparison of 35S:SlmiR-168a and WT plants, with
upregulated/upregulated, downregulated/downregulated,
upregulated/downregulated, downregulated/upregulated by integrated
analysis of miRNA-Seq and mRNA-Seq.
Table S11. miRNA/mRNA pairs in the comparison of35S:rSlAGO1 and WT plants, with upregulated/upregulated,
downregulated/downregulated, upregulated/downregulated,
downregulated/upregulated by integrated analysis of miRNA-Seq and
mRNA-Seq.
Table S12. List of the CTK- and ABA-related genes that were
downregulated in 35S:rSlAGO1 and upregulated in35S:SlmiR-168a .
Figure S1. GO analyses of
the 10 negative miRNA/mRNA pairs identified in the comparison of35S:rSlAGO1 and WT plants by integrated analysis of miRNA-Seq and
mRNA-Seq.
Figure S2. KEGG pathway enrichment analyses of the 10 negative
miRNA/mRNA pairs identified in the comparison of 35S:rSlAGO1 and
WT plants by integrated analysis of miRNA-Seq and mRNA-Seq.
Figure S3. GO analyses of the 2 negative miRNA/mRNA pairs
identified in the comparison of 35S:SlmiR-168a and WT plants by
integrated analysis of miRNA-Seq and mRNA-Seq.
Figure S4. Quantitative real-time PCR validation of P68 in35S:SlmiR-168a , 35S:rSlAGO and WT. The experiments were
repeated three times.
Figure S5. Comparison of morphological changes of root growth
in WT, 35S:SlmiR-168a , and 35S:rSlAGO1 plants under normal
K+ conditions and K+ deficiency
stress after 7 days.