Integrated analysis of mRNA-seq and miRNA-seq data
In order to define all possible miRNA/mRNA interactions, including
positive and negative relationships between miRNA and mRNA expression,
we used ACGT101-CORR 1.1 to construct the miRNA/mRNA regulatory network.
Briefly, we normalised all the sample-matched miRNA and mRNA sequencing
data. Integration of miRNA-seq with mRNA-seq data was then achieved by
integrating profiles of differentially expressed miRNAs and mRNAs with
the addition of differentially expressed miRNA-targeting information.