Integrated analysis of mRNA-seq and miRNA-seq data
In order to define all possible miRNA/mRNA interactions, including positive and negative relationships between miRNA and mRNA expression, we used ACGT101-CORR 1.1 to construct the miRNA/mRNA regulatory network. Briefly, we normalised all the sample-matched miRNA and mRNA sequencing data. Integration of miRNA-seq with mRNA-seq data was then achieved by integrating profiles of differentially expressed miRNAs and mRNAs with the addition of differentially expressed miRNA-targeting information.