Luciferase reporter gene assay
Truncated heparanase promoters (-2000–0, -1000–0, -300–0) were cloned
forward of the luciferase reporter gene in a pGL3 basic vector, which
was co-transfected with PPARγ vectors into 293T cells using
Lipofectamine 2000 (Invitrogen). Luciferase activity was detected with
the Luciferase Reporter Assay System (Promega, Fitchburg, WI, USA), as
previously described (Li et al. , 2019).