OGT2115 prevented loss of intra-islet HS level and reduced
infiltration of immunocytes in STZ mice
To investigate whether the protective effect of OGT2115 on STZ-induced
pancreatic beta cell damage was directly due to restoration of
intra-islet HS levels, HS was stained with Alcian blue dye or HS
antibody. As shown in Fig. 5A and 5B, the intra-islet HS was clearly
lost in the STZ mice. By contrast, the STZ mice treated with either 3
mg/kg or 10 mg/kg OGT2115 showed a significant increase in intra-islet
HS (Fig. 5A and Fig. 5B). Multiple low-dose injections of STZ caused
beta cell death and could induce subsequent infiltration of
F4/80-positive monocytes (Fig. 5A and 5C), CD8 or CD4-positive T cells
(Fig. 5A and 5D). Quantitative analysis of F4/80, CD8, and CD4 positive
cell numbers demonstrated a notable decrease in the infiltration of
immunocytes in the islets of 10 mg/kg OGT2115-treated STZ mice when
compared with vehicle-treated STZ mice (Fig. 5C and Fig. 5D).