OGT2115 did not protect pancreatic beta cells from STZ -induced dysfunction in vitro
We used the mouse insulinoma MIN6 cell line and primary isolated murine islets to test whether OGT2115 had a protective effect in cultured beta cells in vitro . The MTT assay showed that a high dosage of OGT2115 (more than 10 μM) could significantly disrupt the viability of MIN6 cells (IC50=10.31 μM, Fig. 6A). Concentrations of up to 1 μM OGT2115 were used in further in vitro experiments. STZ reduced the viability of MIN6 cells after a 24 h incubation. However, co-incubation with OGT2115 did not prevent the damage by STZ (Fig. 6B) on MIN6 cells. Likewise, OGT2115 did not protect against STZ-induced damage in the GSIS of cultured islets (Fig. 6C). The STZ- induced reduction in mRNA level of genes involved in insulin expression and secretion, includinginsulin , Pdx-1 and Mafa , were not improved by OGT2115 treatment of cultured islets (Fig. 6D).