2.8. In vivo studies in full-thickness skin defect SD rat model
All SD rats (60d postnatal) used in this study were purchased from the
animal center of Guangdong province (No. 44007200069979) and caged under
controlled room temperature, humidity, and light (12/12-h light-dark
cycle) with access to water and food ad libitum. The experimental
protocols used in this study were approved by the Institutional Animal
Care and Use Committee of Jinan University (Approval number: 2019228).
All experiments were conducted according to the guidelines for animal
care and use of China, and they were approved by the animal ethics
committee of the Chinese Academy of Medical Science. Establish a
full-thickness skin defect model and evaluate role of the RHC, EGF,
RHC/EGF freeze-dried dressing in the wound healing. Female SD rats (200
± 20g, n = 80) were randomly assigned to four groups: (1) Control group
(treated with physiological saline), (2) EGF group, (3) RHC group, (4)
RHC/EGF group. In short, rats were anesthetized with an intraperitoneal
injection of 10% chloral hydrate, and their backs were shaved and
cleaned with ethanol. A whole-skin defect model was prepared with a
1.8-cm diameter ring drill to compress a circular mark (2.54
cm2), and then the skin was cut off along the mark.
Wounds were treated with physiological saline, EGF, RHC or RHC/EGF
freeze-dried dressing.