3.8. RHC/EGF promotes neovascularization of wound skin
Neovascularization is necessarily
required for normal tissue development, particularly to provide oxygen
and nutrition to granulation tissues. CD31 is found on the surface of
endothelial cells and is widely used as a marker of
angiogenesis.(Camila et al.) VEGF, as a
marker of angiogenesis, can enhance
vascular permeability and promote the formation of capillaries in new
blood vessels.(Jelena et al.) Damaged
skin was collected on days 3 and 14 and the expression of CD31 and VEGF
in vascular network was assessed by IHC (Fig. 6). After treatment for 3
or 14 d, expression of CD31 was significantly increased in EGF and
RHC/EGF groups compared with control and RHC groups. At the same time
points, VEGF expression exhibited the same
trend. As angiogenesis biomarkers,
upregulation of CD31 and VEGF demonstrates that RHC/EGF can accelerate
wound healing and repair. Therefore, RHC/EGF freeze-dried can promote
wound healing by promoting angiogenesis. Local application of growth
factors such as bFGF, EGF, and VEGF are the most common methods to
promote wound healing by promoting
angiogenesis.(Qu et al.;
Xue, Zhao, Lin, & Jackson) However, skin
defect repair is a complex process that requires the synergistic effects
of cell adhesion, spread, migration, and angiogenesis. As a result, many
of these growth factors fail to achieve complete wound healing. In
addition, collagen has a good role in promoting cell adhesion and
migration, but has the disadvantages of poor solubility and easy to
cause immune response Therefore, we
prepared RHC/EGF freeze-dried while promoting adhesion, diffusion,
migration, and angiogenesis. In summary, RHC/EGF promotes cell adhesion,
proliferation, migration, and angiogenesis in vitro. In vivo, RHC/EGF
freeze-dried accelerate wound healing by enhancing re-epithelialization,
promoting collagen deposition and angiogenesis. Therefore, RHC/EGF
freeze-dried may be a promising dressing for wound healing.