2.9 Macroscopic evaluation of wounded tissues
For measurement of wound closure percentage, wounds were photographed at
0, 3, 7, 10, 14, and 21 d post-wounding and analyzed by using ImageJ
software. The wound area was measured by the limits of grossly evident
epithelialization. The wound closure percentage at each time point was
derived by the following formula: [(Initial wound size-current wound
size)/initial wound sizeĆ100].