Figure 6. Piperine relieves inflammation and myelin destruction
in EAE model
(A) Representative histological images of spinal cord sections from
piperine- or vehicle- treated EAE mice in preventive model. The sections
were prepared on day 22 after MOG’s immunization. Hematoxylin and eosin
(H&E) staining for assessing of inflammation (left) and luxol fast-blue
(LFB) staining for assessing of demyelination (right).
(B) BBB disruption was imaged using BSA-Cy5.5 at Ex/Em=680/720 nm
(left). Mice were i.v. injected with BSA-Cy5.5 (50 mg/kg) and subjected
to fluorescence imaging at 6 h post-injection. Quantitative results of
total fluorescent counts of BSA-Cy5.5 in regions of interest (right).
Values are represented as mean ± SEM (n=3). Data are shown as mean ± SEM
and Student’s t-test is performed, *P<0.05.
(C) Myelin imaging was performed with a near-infrared dye,
3,3-diethylthiatricarbocyanine iodide (DBT) (left). DBT dye (0.3 mg/kg)
were administered to mice by i.v. injections into the tail vein. The
fluorescence imaging of the mouse myelin was acquired 5 min
post-injection of DBT with Ex/Em=745/800 nm. Quantitative results of
fluorescent intensity of DBT in mice (right). Values are represented as
mean ± SEM (n=3). Data are shown as mean ± SEM and Student’s t-test is
performed, *P<0.05.
(D-E) Flow cytometry analysis of T lymphocytes. Splenocytes were
incubated with APC-CD8+ (A) and
FITC-CD4+ (B) antibodies for 2 h at 4 °C in the dark,
respectively. Flow cytometry analysis was performed on a BD FACS Caliber
equipped with Cell Quest software (BD Biosciences, NJ, USA).
Quantitative data of CD8+ and CD4+population were presented in right, respectively. Data are shown
as mean ± SEM and Student’s t-test is performed, ***P< 0.001.