EXPERIMENTAL APPROACH
Biochemical and Biophysical methods, including enzymatic kinetic
analysis, thermofluor assay, isothermal titration calorimetry and X-ray
crystallography were used to assess DHODH inhibition. The
immunomodulatory activity was assessed by using concanavalin a-triggered
T-cell assay and mixed lymphocyte reaction assay. MOG-induced
experimental allergic encephalomyelitis (EAE) was used to assess thein vivo therapeutic effects. Myelin destruction and blood–brain
barrier (BBB) was evaluated via in vivo imaging