Figure 5. Piperine reduces clinical score and severity of EAE
(A) Schematic diagram of EAE model with piperine treatment beginning at
day 10.
(B) Daily mean EAE clinical score of C57BL6 mice (n = 8) on preventive
treatment with vehicle, piperine (10 mg/kg and 30 mg/kg), or A771726 (10
mg/kg). Drugs was administered through daily i.p. injection at day 10
after EAE induction. Data are shown as mean ± SEM and Student’s t-test
is performed, **P<0.01, ***P< 0.001.
(C) The sum of EAE scores for each individual mouse in different groups
in b . Data are shown as mean ± SEM and Student’s t-test is
performed,
**P<0.01,
***P < 0.001.
(D) The body weight was recorded daily during the EAE course
(day12-day28). Data are shown as mean ± SEM and Student’s t-test is
performed, **P<0.01, ***P< 0.001.
(E) Schematic diagram of EAE model with piperine treatment initiating at
day 20.
(F) Daily mean EAE clinical score of C57BL6 mice (n = 8) on therapeutic
treatment with vehicle, piperine (30 mg/kg), or A771726 (10 mg/kg).
Drugs was administered through daily i.p. injection at day 20 after EAE
induction. Data are shown as mean ± SEM and Student’s t-test is
performed, **P<0.01.
(G) The sum of EAE scores for each individual mouse in different groups
in (F). The piperine-treated mice showed a notably decrease in the total
scoring. Data are shown as mean ± SEM and Student’s t-test is performed,
*P<0.05, ***P< 0.001.
(H) The body weight was recorded daily during the EAE course
(day14-day30). Data are shown as mean ± SEM and Student’s t-test is
performed, *P<0.05, ***P< 0.001.