MOG35–55 induced chronic progressive EAE model
in C57BL/6 mice
10-12
weeks old female C57BL/6 mice were purchased from Shanghai Laboratory
Animal Center. All animal care and experimental procedures in this study
were conducted in compliance with the protocol approved by the
institutional Animal Care and Use Committee at East China University of
Science and Technology. Mice were housed for 7 days in a flow cabinet
with a 12 h light/dark cycle and fed with standard rodent chow and tap
water ad libitum. For EAE induction, mice were
immunized
in the flank with 100 µg MOG35-55 peptide dissolved in Complete Freund’s
Adjuvant (CFA, including extract 4 mg/ml M. tuberculosis H37Ra; Beijing
Biolead Biology Sci & Tech)(Najm et al., 2015). The 200 ng/mouse
pertussis toxin (PTX) was injected on day 0 and day 2. Treatment with
PIP or vehicle started after immunization after 12 days and kept 17
days. Clinical symptoms scoring for MOG35-55 induced EAE was evaluated
as follows: score 0, no obvious changes in motor functions; score 0.5,
distal paralysis of the tail; score 1, complete tail paralysis; score
1.5, mild paresis of one or both hind legs; score 2, severe paresis of
hind legs; score 2.5, complete paralysis of one hind leg; score 3,
complete paralysis of both hind legs.