Flow Cytometry Analysis
Preparation of individual cell suspensions from EAE mouse was performed as described before. Splenocytes were resuspended in FACS buffer (PBS, pH7.4, supplemented with 1 mM EDTA and 2 % FBS) and then incubated with FITC-CD4+ (mouse) and APC-CD8+ (mouse) antibodies for 2 h at 4 °C in the dark, respectively. Flow cytometry analysis was performed on a BD FACS Caliber equipped with Cell Quest software (BD Biosciences, NJ, USA).