Figure 5. Piperine reduces clinical score and severity of EAE
(A) Schematic diagram of EAE model with piperine treatment beginning at day 10.
(B) Daily mean EAE clinical score of C57BL6 mice (n = 8) on preventive treatment with vehicle, piperine (10 mg/kg and 30 mg/kg), or A771726 (10 mg/kg). Drugs was administered through daily i.p. injection at day 10 after EAE induction. Data are shown as mean ± SEM and Student’s t-test is performed, **P<0.01, ***P< 0.001.
(C) The sum of EAE scores for each individual mouse in different groups in b . Data are shown as mean ± SEM and Student’s t-test is performed, **P<0.01, ***P < 0.001.
(D) The body weight was recorded daily during the EAE course (day12-day28). Data are shown as mean ± SEM and Student’s t-test is performed, **P<0.01, ***P< 0.001.
(E) Schematic diagram of EAE model with piperine treatment initiating at day 20.
(F) Daily mean EAE clinical score of C57BL6 mice (n = 8) on therapeutic treatment with vehicle, piperine (30 mg/kg), or A771726 (10 mg/kg). Drugs was administered through daily i.p. injection at day 20 after EAE induction. Data are shown as mean ± SEM and Student’s t-test is performed, **P<0.01.
(G) The sum of EAE scores for each individual mouse in different groups in (F). The piperine-treated mice showed a notably decrease in the total scoring. Data are shown as mean ± SEM and Student’s t-test is performed, *P<0.05, ***P< 0.001.
(H) The body weight was recorded daily during the EAE course (day14-day30). Data are shown as mean ± SEM and Student’s t-test is performed, *P<0.05, ***P< 0.001.