Whole genome sequencing
For genome sequencing, the strain ATA-11A-B (=CBS
18375T) was cultured in 15 ml of YPD broth at 25°C for
72 hours. Genomic DNA extraction was performed using the Qiagen
Genomic-tip 20/G kit (Qiagen, Germany), following the previously
described method by Nespolo et al. in 2020 (Nespolo et al. ,
2020). The extracted DNA was quantified using the Invitrogen™ Qubit™ 4
Fluorometer (cat. no. Q33226), and a genomic DNA library was prepared
using the Illumina DNA-prep kit, following the manufacturer’s protocol.
Subsequently, the libraries were sequenced using the Illumina NextSeq500
with a Mid-Output Kit, generating 150-bp paired-end reads, at the
sequencing facility of the University of Santiago de Chile.