Fig. 7 COL13 promotes the interaction between COL3 and COP1.
(a) Yeast three-hybrid analysis of COP1-COL3 interaction in the presence
of COL13. Normalized Miller units were calculated as a ratio of
α-galactosidase activity in yeast. Additionally, normalized Miller units
are reported separately for yeast grown on media with or without 1 mM
methionine (Met), corresponding to induction (-Met) or repression (+Met)
of Met25 promoter-driven COL13 expression, respectively. Means
and standard errors of the means for three biological repetitions are
shown. Lower-case letters indicate significant differences in
α-galactosidase. (b) A model representing the HY5-COL3-COL13 regulatory
chain and COP1-dependent COL3-COL13 feedback pathway in the regulation
of hypocotyl elongation.