Table S2 Probes used in EMSA assay.
Fig. S1 COL13 regulates hypocotyl elongation under red-light conditions. (a) Scheme of the Arabidopsis COL13 gene (AT2G47890) showing the T-DNA insertion position. (b) Identification of the sulfonamide resistance gene (SUL) in the col13 mutant by PCR. (c) The hypocotyl lengths of the wild type (WT), COL13 RNAi, and col13 seedlings under different light conditions. Error bars indicate the standard deviations (n >15). Lower-case letters indicate significant differences (P < 0.05).
Fig. S2 Hypocotyl lengths of the indicated genotypes were measured at the 5th day. Error bars indicate SD (n >15). Asterisks indicate that hypocotyl lengths in 35S:COL-HA and 35S:COL13-GFP are significantly different with Col-0 under red light (P < 0.05).
Fig. S3 COP1 can interact with COL3, but not COL13. (a) COL3-CFP and COP1-YFP co-localized to the nucleus in protoplasts under both light and dark conditions. (b) FRET between CFP-COL3 and YFP-COP1 analyzed by acceptor bleaching in the nucleus. The top panels in b show a representative pre-bleach nucleus co-expressing YFP-COP1 and CFP-COL3 excited with either a 514 or a 405 nm laser in light and dark, resulting in emission from YFP (yellow) or CFP (blue), respectively. The bottom panels in (b) show the same nucleus after bleaching following excitation with a 514 or a 405 nm laser. The relative intensities of both YFP and CFP were measured once before and twice after bleaching, as indicated in (c) and (d). (e) COL13-CFP and COP1-YFP co-localized to the nucleus in protoplasts in light and dark. (f) FRET between CFP-COL13 and YFP-COP1 analyzed by acceptor bleaching in the nucleus. The top panels in f show a representative pre-bleach nucleus co-expressing YFP-COP1 and CFP-COL13 excited with either a 514 or a 405 nm laser in light and dark, resulting in emission from YFP (yellow) or CFP (blue), respectively. The bottom panels in f show the same nucleus after bleaching following excitation with a 514- or a 405-nm laser. The relative intensities of both YFP and CFP were measured once before and twice after bleaching, as indicated in (g) and (h).