Plant transformation
Constructs in binary vectors were introduced into theAgrobacterium tumefaciens strain LBA4404 and transformed intoArabidopsis wild type (WT) or mutant plants by the floral-dip method (Clough & Bent, 1998). Approximately 30 T1 transgenic plants for each transgene were screened on MS medium supplemented with the appropriate antibiotics, and phenotypic analyses were performed on T2 or later generations.