Hypocotyl Experiments
The light-response assays were performed as previously described with
some changes (Datta et al., 2006). Plates were treated at 4 °C for 72 h
and then moved to white light for 10 h to induce uniform germination.
After that, the plates were transferred to different light conditions
(dark, white, red, and blue light) and incubated at 22 °C for 3–6 d for
hypocotyl measurement. Red and blue lights were generated by light
emission diodes at 670 and 470 nm, respectively, (model E-30LED;
Percival Scientific). The light intensity was approximately 100
μmol/m2 s ppfd. The hypocotyl lengths of seedlings
were measured and counted using ImageJ software.