Introduction
Through their lifecycle, plants experience environmental conditions that
vary on timescales from seconds to seasons. Arabidopsis thalianais typically a winter annual, germinating in the autumn and persisting
through the winter, prior to flowering in spring
(Grimeet al. , 1988). Across this time-period, mean daily temperatures
may vary by 20°C or more. To optimise survival and growth, plants
acclimate to changes in temperature, altering their investment in
different processes to suit the conditions experienced (Ruelland,
Vaultier, Zachowski, & Hurry, 2009; Walters, 2005). The process of
acclimation can involve both structural changes, with tissues differing
when developed in different conditions, but also relatively rapid
(days-weeks) dynamic responses, which tracks changes in the environment
(Athanasiou, Dyson, Webster, & Johnson, 2010; Walters, 2005). Changes
in both light intensity and temperature are known to trigger acclimation
(Athanasiou et al. , 2010; Dyson et al. , 2015; Dysonet al. , 2016; Huner et al. , 1993; Savitch et al. ,
2001; Stitt & Hurry, 2002; Strand, Hurry, Gustafsson, & Gardestrom,
1997; Walters & Horton, 1994).
Exposure to low temperature triggers a complex array of acclimation
responses. A significant amount of work in plant cold responses has
focused on the acquisition of freezing tolerance (see Knight & Knight,
2012 for a review). In addition, it is recognised that plants can
optimise their metabolism to suit changing conditions. For example,
acclimation of both photosynthesis (Huner et al. , 1993; Strandet al. , 1997; Strand et al. , 1999) and respiration
(Armstrong, Logan, Tobin, O’Toole, & Atkin, 2006; Talts, Pärnik,
Gardeström, & Keerberg, 2004) to low temperature are seen, these being
metabolic processes which are easily monitored in vivo . A
prominent feature of metabolic acclimation is an increase in the
capacity of metabolism, with enzyme and metabolite concentrations
increasing to compensate for the loss of activity at low temperature.
This requires the coordination of gene expression across multiple
cellular compartments, including retrograde and/or anterograde signals
between the nucleus, chloroplast and mitochondrion (Fey, Wagner,
Brautigam, & Pfannschmidt, 2005). To date, little is known about either
the sensing or the signalling pathways involved in metabolic
acclimation.
Acclimation of photosynthesis to low temperature has previously been
studied in a range of species, including Arabidopsis. In the short term,
low temperature decreases the rate at which sucrose is synthesised and
exported from the leaf, with the enzyme sucrose phosphate synthase (SPS)
receiving particular attention. Limitations in flux through SPS result
in the accumulation of phosphorylated metabolites (Hurry, Strand,
Furbank, & Stitt, 2000). This is thought to lead to depletion of the
cellular concentration of inorganic phosphate (Pi) which in turn limits
the export of triose phosphates from the chloroplast. This inhibits
photosynthesis, as the regeneration of Pi in the chloroplast is
necessary for ATP synthesis. Over relatively short periods (days)
increased expression of SPS removes the limitation in sucrose synthesis.
Hurry et al.
(2000)
provided evidence, based on the responses of different mutants with
altered phosphate content, leading to the hypothesis that Pi depletion
provided a signal for cold acclimation of SPS content.
Recently, we re-examined the responses of Arabidopsis to low temperature
(Dysonet al. , 2016). Using non-targeted metabolomics, we identified the
diel accumulation of the organic acid fumaric acid (predominantly
present in the anionic form, fumarate) as important in the response to
cold. We showed that the accumulation of fumarate, which requires the
presence of a cytosolic isoform of the enzyme fumarase
(Pracharoenwattanaet al. , 2010), is a specific response to temperature. Plants of a
mutant lacking this enzyme, fum2 , not only failed to accumulate
fumarate over the photoperiod, they also accumulated higherconcentrations of phosphorylated intermediates. Crucially, whilst
wild-type Arabidopsis plants increase photosynthetic capacity in
response to low temperature, fum2 plants do not. This suggests
that Pi deficiency alone is not sufficient to trigger photosynthetic
acclimation.
Here, we have used a combined metabolic and proteomic approach, together
with physiological analyses to dissect the acclimation processes
occurring in Arabidopsis in response to low temperature. We focus on
dynamic acclimation responses to cold in leaves developed at higher
temperature. Our results show that fumarate accumulation is important
for a wide range of metabolic acclimation responses to cold. Based on
metabolic modelling, constrained using experimental data, we propose
that changes in the pathway of carbohydrate export from the chloroplast
link to fumarate accumulation at low temperature and provide a mechanism
for retrograde signalling from the chloroplast driving acclimation.