Figure 1 a. Sucrose content in rice grains at 5d, 10d, 15d, 20d and the mature stage after light treatment in 2018 and 2019;b.Contents of ABA in rice grains at 5d, 10d, 15d, and 20d after light treatment in 2019.
Figure 2 a. The number of circRNAs shared between different light treatments and different development times: all developmental stages (purple), the treatment and control at 5 d (orange), treatment and control at 10 d (green), three periods of control (red), and three periods of low light (pink). b . The sequences of all circRNAs constitute a specific source. c. The numbers of differentially expressed circRNAs were compared in the control at 5d and 10d, and at 5d and 10d after exposure to low light.
Figure 3 a. Networked view of the differentially expressed circRNAs targeting miRNAs. Blue represents miRNA, green represents circRNA that targets more than two miRNAs, and yellow represents less than two circRNAs. b. Validation of the differentially expressed circRNAs by real-time PCR assay. See Table 1 for the circRNA names. Means of samples denoted with the “*” are significantly different at P < 0.05 according to the t-test compared to the control after 5 d.
Figure 4 Possible regulatory mechanism involving differentially expressed circRNAs and their target genes in rice grains under weak light.