Contributions of plasticity and evolution to total metabolomic
changes in time
For the positive ion mode, ancestral plasticity had about an equal
contribution to the total metabolomic change compared to the
evolutionary components during both transitions in fish predation
(47.3% in the first transition and 55.9% in the second transition,
Figure 5a-b). Of the two evolutionary components, the contribution of
evolution of plasticity was larger (31.0% and 24.6%) compared to
constitutive evolution (21.7% and 19.4%) during both transitions. The
results were highly similar for the negative ion mode: ancestral
plasticity had about an equal contribution compared with evolution
during both transitions (46.5% and 48.8%), and the evolution of
plasticity contributed more (30.4% and 30.0%) than constitutive
evolution (23.1% and 21.2%, Figure 5c-d).