Figure Caption
Figure 1. DNA metabarcoding constraints and proposed solutions.
References
Baird DJ, Hajibabaei M
(2012) Biomonitoring 2.0: a new paradigm in ecosystem assessment made
possible by next-generation DNA sequencing. Molecular Ecology ,21 , 2039-2044.
Duke EM, Burton RS
(2020) Efficacy of metabarcoding for identification of fish eggs
evaluated with mock communities. Ecology and evolution ,10 (7),
3463-3476.
Deagle BE, Thomas AC,
McInnes JC et al. (2018) Counting with DNA in metabarcoding
studies: How should we convert sequence reads to dietary data?Molecular Ecology , 28 (2),
391-406.
Lamb PD, Hunter E,
Pinnegar JK, et al. (2019) How quantitative is metabarcoding: A
meta‐analytical approach. Molecular Ecology , 28 (2),
420-430.
Leese F, Bouchez A,
Abarenkov K, et al. (2018) Chapter Two – Why We Need Sustainable
Networks Bridging Countries, Disciplines, Cultures and Generations for
Aquatic Biomonitoring 2.0: A Perspective Derived From the DNAqua-Net
COST Action, in: Bohan DA, Dumbrell AJ, Woodward G, Jackson M (Eds.),Advances in Ecological Research, Next Generation Biomonitoring:
Part 1 . Academic Press, pp. 63–99.
Mariac
C, Renno JF, Carmen Garcia-Davila et al. (2021) Species-level
ichthyoplankton dynamics for 97 fishes in two major river basins of the
Amazon using quantitative metabarcoding.
Molecular ecology, 30,
XXX-XXX.
Mariac C, Vigouroux Y,
Duponchelle F et al. (2018) Metabarcoding by capture using a
single COI probe (MCSP) to identify and quantify fish species in
ichthyoplankton swarms. PLoS One , 13(9),
e0202976.
Milan DT, Mendes IS,
Damasceno JS et al. (2020) New 12S metabarcoding primers for
enhanced Neotropical freshwater fish biodiversity assessment.Scientific reports , 10 ,
17966.
Ratcliffe FC, Uren
Webster TM, Rodriguez‐Barreto D et al. (2021) Quantitative
assessment of fish larvae community composition in spawning areas using
metabarcoding of bulk samples. Ecological Applications ,31 (3), e02284.
Santos RP, Melo BF,
Yazbeck GM et al. (2021). Diversification of Prochilodusin the eastern Brazilian Shield: Evidence from complete mitochondrial
genomes (Teleostei, Prochilodontidae). Journal of Zoological
Systematics and Evolutionary Research , 59 (5),
1053-1063.
Thomas AC, Deagle BE,
Eveson JP, Harsch CH, & Trites AW (2016) Quantitative DNA
metabarcoding: improved estimates of species proportional biomass using
correction factors derived from control material. Molecular
ecology resources , 16 (3),
714-726.