The procedure of injection in rats
As it was described in detail earlier , PITS-composition was injected through catheter. Rats were catheterized in the jugular vein and were able to freely move in a system with swivels (DiLab 100,014, USA). One day before the experiment, 100 μl of heparin (50 IU/ml) was injected into the catheter. On the next day, 1 ml of PITS-composition or vehicle (physiological solution) was injected into the catheter in accordance with protocol of the experiment. After the injection of drugs, the catheter was filled with 100 μl of heparin (50 IU/ ml) to flush the system and prevent clotting in the catheter.
Xylazine (Interchemie, Netherlands), 2% dissolved in physiological solution, was administered intramuscularly (0,40 ml  per 1 kg body weight).