The procedure of injection in rats
As it was described in detail earlier , PITS-composition was injected
through catheter. Rats were catheterized in the jugular vein and were
able to freely move in a system with swivels (DiLab 100,014, USA). One
day before the experiment, 100 μl of heparin (50 IU/ml) was injected
into the catheter. On the next day, 1 ml of PITS-composition or vehicle
(physiological solution) was injected into the catheter in accordance
with protocol of the experiment. After the injection of drugs, the
catheter was filled with 100 μl of heparin (50 IU/ ml) to flush the
system and prevent clotting in the catheter.
Xylazine (Interchemie, Netherlands), 2% dissolved in physiological
solution, was administered intramuscularly (0,40 ml per 1 kg body
weight).