Preparation of PITS-composition
The procedure for preparing the PITS-composition was described earlier . Shortly, to prepare the composition we used the following substances obtained from Sigma-Aldrich and PMBio: 1. Propranolol hydrochloride 5 mg/kg of animal weight; 2. Ivabradine hydrochloride 5 mg/kg of animal weight; 3. Diphenhydramine hydrochloride 5 mg/kg of animal weight; 4. Propylthiouracil 5 mg/kg of animal weight; 5. Serotonin hydrochloride 5 mg/kg of animal weight; 6. Magnesium sulfate 30 mg/kg of animal weight; 7. Reserpine 1 mg/kg of animal weight; 8. Periciazine, 4 mg/kg of animal weight; 9. Lipofundin® MCT/LCT 20% 2.0 ml/kg of animal weight. 10. The emulsion was saturated with inert gas xenon 0.54 ± 0.09 ml/ml (gas/liquid).