Estimation of Catalase activity
Catalase activity was measured according to Aebi et al. (1984). The composition of enzyme assay mixture consisted of 100 µL enzyme extract, 100 mM phosphate buffer (pH 7.0) and 30 mM H2O2 in a total volume of 1.5 ml. The H2O2 was added in the last and absorbance was measured by UV/VIS spectrophotometer at 240 nm. The disappearance of substrate (H2O2) by enzymatic breakdown was monitored by decrease in absorbance till 3 minutes.