Plant material and phenotyping
The MAGIC population was derived from a cross between eight parental
lines that belong to Solanum. lycopersicum and Solanum
lycopersicum cerasiforme groups. More details about the population
development can be found in Pascual et al. (2015). Briefly, the
population was composed of about 400 8-way MAGIC lines that underwent
three generations of selfing before greenhouse evaluations were carried
out. In this study, a subset of 241 to 397 lines was grown in each
environment (Supplemental Table 1).
The full genome of each parental line was re-sequenced and their
comparison with the reference tomato genome (‘Heinz 1706’) yielded 4
millions SNPs (Causse et al., 2013). From these polymorphisms, a genetic
map of 1345 discriminant SNPs was developed (Pascual et al., 2015) and
used in the present study for the QTL analysis.