Expression Patterns of CsWRKY20 and CsLecRK6.1gene in leaves
As shown in Fig1, the transcripts of the CsLecRK6.1 andCsWRKY20 genes responding to different time points were detected
in resistant, moderately resistant and high susceptible genotypes but at
different levels. In the Ramezz and Baby, CsLecRK6.1 transcript
levels was strongly up-regulated as early as 24 hpi (3.02 and 2.32 fold)
which gradually increased and reached a peak at 72 hpi (7.86 and 7.94
fold), respectively (Fig2.a). In contrast, high susceptible Mini 6-23
and Extrem showed consistent and low expression of CsLecRK6.1until 72 h after infection. The gene, CsWRKY20 , had increased
expression levels between 0 to 48 hpi and then declined from 48 hpi to
72 hpi, although in Ramezz and Baby was still higher than the transcript
expression in Mini 6-23 and Extrem (Fig2.b). CsWRKY20 transcript
accumulation in leaves of Ramezz showed the strongest, 6.78-fold
enhancement at 48 hpi compared with the control (Fig2.b). The
differential expressions of these candidate transcripts may lead to the
difference in resistance level among different genotypes and tissues.