Chemicals
Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Euroclone
(MI, Italy); L-glutamine, penicillin-streptomycin mixture, and AdipoRed
Assay Reagent were purchased from Lonza (East Rutherford, NJ, USA); MEM
non-essential amino acid solution M7145, palmitic acid (PA; C16:0),
oleic acid (OA; C18:1 cis-9), bovine serum albumin (BSA) Cohn fraction
V, Hydroxytyrosol (HT) and Quercetin (Que) were purchased from Merck
(Darmstadt, Germany); Fetal Bovine Serum (FBS, Gibco), charcoal-stripped
FBS, Hoechst 33342 fluorescent nuclear dye and ethidium homodimer-1
(EthD-1) were purchased from Thermo Fisher Scientific (Waltham, MA,
USA); ROS-ID Total ROS/Superoxide Detection Kit (ENZ-51010) was
purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA).