3.4 | Non-interference of mAb-EspB-B7 with the
EspB-EspD interaction
To examine the ability of mAb-EspB-B7 to interfere with the known
EspB-EspD interaction (Luo & Donnenberg, 2011), we examined the ability
of EspB to co-elute with EspD in the absence or the presence of
mAb-EspB-B7 (100 or 200 nM). We observed that the presence of
mAb-EspB-B7 did not affect the co-elution of EspB with EspD (Figure 4),
suggesting that mAb-EspB-B7 does not interfere with the EspB-EspD
interaction. Low non-specific binding of EspB to the Ni-NTA beads was
observed in the negative control (a sample that did not express
EspD- 35His).