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Dissolved-oxygen feedback control fermentation for enhancing β-carotene in engineered Yarrowia lipolytica
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  • Peng Jun Lv,
  • Shan Qiang,
  • Liang Liu,
  • Ching Yuan Hu,
  • Yonghong Meng
Peng Jun Lv
Shaanxi Normal University
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Shan Qiang
Xi’an Healthful Biotechnology Co.
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Liang Liu
Shaanxi Normal University
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Ching Yuan Hu
Shaanxi Normal University
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Yonghong Meng
Shaanxi Normal University
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Abstract

β-carotene is an indispensable nutrient for human health and its global consumer demand is increasing. In order to promote the market supply and volumetric productivity of β-carotene using engineered Yarrowia. lipolytica strain. The fed-batch fermentation and DO-stat fed-batch fermentation were carried out. 73.5g/L biomass and 0.3g/L β-carotene were obtained in fed-batch. 94g/L biomass and 0.4g/L β-carotene were harvest during DO-stat fed-batch fermentation. The results of intracellular energy showed that more ATP and NADP+/NADPH were produced in DO-stat fed-batch fermentation as compared to that under fed-batch fermentation. Moreover, three kinetic models showed that the higher μm, Yx/s, and Yp/s values were achieved for DO-stat fed-batch fermentation. In this study, the DO-stat strategy was first reported for the β-carotene produce and the obtained results showed that it is a promising strategy in the industrialized β-carotene production.