Dissolved-oxygen feedback control fermentation for enhancing β-carotene
in engineered Yarrowia lipolytica
β-carotene is an indispensable nutrient for human health and its global
consumer demand is increasing. In order to promote the market supply and
volumetric productivity of β-carotene using engineered Yarrowia.
lipolytica strain. The fed-batch fermentation and DO-stat fed-batch
fermentation were carried out. 73.5g/L biomass and 0.3g/L β-carotene
were obtained in fed-batch. 94g/L biomass and 0.4g/L β-carotene were
harvest during DO-stat fed-batch fermentation. The results of
intracellular energy showed that more ATP and NADP+/NADPH were produced
in DO-stat fed-batch fermentation as compared to that under fed-batch
fermentation. Moreover, three kinetic models showed that the higher μm,
Yx/s, and Yp/s values were achieved for DO-stat fed-batch fermentation.
In this study, the DO-stat strategy was first reported for the
β-carotene produce and the obtained results showed that it is a
promising strategy in the industrialized β-carotene production.