List of Figures
Figure 1. Scheme of preparation of Dopa-incorporated
bioengineered MAP (bMAP) proteins. In vivo residue-specific
incorporation of Dopa (A) without coexpression of any AARS, or with
coexpression of (B) Dopa-specific Methanococcus jannascii TyrRS
(MjTyrRS) or (C) E. coli TyrRS (EcTyrRS)
Figure 2. Protein gels of expressed and purified bMAP samples
stained with Coomassie blue (A) or NBT (B). Lanes are denoted as
molecular weight markers (Mw), cell lysate before induction (BI), cell
lysate after induction (AI), purified bMAP (E), Dopa-bMAP without
over-expression of any AARS (Control), or Dopa-bMAP with co-expression
of either MjTyrRS-Dopa (+MjTyrRS) or EcTyrRS(+EcTyrRS). The protein
bands of bMAP samples are marked with an arrow.
Figure 3. MALDI-TOF MS spectra of bMAP samples. (A) Dopa-free
bMAP, (B) Dopa-bMAP_Control, (C) Dopa-bMAP_MjTyrRS, and (D)
Dopa-bMAP_EcTyrRS
Figure 4. Production yields of purified Dopa-bMAP samples
without any AARS (Control) with either MjTyrRS-Dopa (+MjTyrRS) or
EcTyrRS (+EcTyrRS). Experiments were performed in triplicates (error
bars: standard deviations, *: P < 0.05 (two-tailed student’s t
test)).