List of Figures
Figure 1. Scheme of preparation of Dopa-incorporated bioengineered MAP (bMAP) proteins. In vivo residue-specific incorporation of Dopa (A) without coexpression of any AARS, or with coexpression of (B) Dopa-specific Methanococcus jannascii TyrRS (MjTyrRS) or (C) E. coli TyrRS (EcTyrRS)
Figure 2. Protein gels of expressed and purified bMAP samples stained with Coomassie blue (A) or NBT (B). Lanes are denoted as molecular weight markers (Mw), cell lysate before induction (BI), cell lysate after induction (AI), purified bMAP (E), Dopa-bMAP without over-expression of any AARS (Control), or Dopa-bMAP with co-expression of either MjTyrRS-Dopa (+MjTyrRS) or EcTyrRS(+EcTyrRS). The protein bands of bMAP samples are marked with an arrow.
Figure 3. MALDI-TOF MS spectra of bMAP samples. (A) Dopa-free bMAP, (B) Dopa-bMAP_Control, (C) Dopa-bMAP_MjTyrRS, and (D) Dopa-bMAP_EcTyrRS
Figure 4. Production yields of purified Dopa-bMAP samples without any AARS (Control) with either MjTyrRS-Dopa (+MjTyrRS) or EcTyrRS (+EcTyrRS). Experiments were performed in triplicates (error bars: standard deviations, *: P < 0.05 (two-tailed student’s t test)).