2.2 Medium
The liquids such as MS, ½ MS, B5 (as Conventional culture media) and
water were used to evaluate the performance of the mist generation
system. The culture media were prepared according to the specific
protocols, including macro, micro, vitamins, and iron components, with
15gr of sugar per liter and an adjusted pH of 5.8. Due to possible
contamination, the culture media was autoclaved at a temperature of\(121\ C\ \)and pressure 1 bar to prevent any undesirable changes of the
physicochemical properties. Sufficient medium was supplied and stored in
the refrigerator during the experiment to prevent any contamination.