Synthesis of FeCo@SiO2-SS-PEI- PLA -PEG-FA and
FeCo@SiO2-SS-PEI-PLA-PEG-Glu and FeCo@SiO2-SS-PEI-PLA-PEG-Glu-FA
FeCo@SiO2-SS-PEI, acrylate-PLA-PEG-FA and acrylate-PLA-
PEG-Glu were dissolved in 6ml of chloroform, separately. The chloroform
solution of acrylate-PLA-PEG-FA and acrylate-PLA-PEG-Glu was added
dropwise into FeCo@SiO2-SS-PEI solution separately. Then
the mixture was stirred for 24 h at 45 °C and stirred for 24 h. The
resulting nanoparticles were washed with distilled water and ethanol,
then the dispersion was centrifuged at 11068 ×g for 30 minutes and the
supernatant was removed. The nanoparticles were lyophilized. To
synthesize FeCo@SiO2-SS-PEI-PLA-PEG-Glu-FA nanoparticles, the same
amounts of acrylate-PLA-PEG-FA and acrylate-PLA-PEG-Glu were added into
FeCo@SiO2-SS-PEI solution. Then the FeCo@SiO2-SS-PEI-PLA-PEG-Glu-FA
nanoparticles were synthesized as protocol mentioned above.