Synthesis of FeCo@SiO2-SS-PEI- PLA -PEG-FA and FeCo@SiO2-SS-PEI-PLA-PEG-Glu and FeCo@SiO2-SS-PEI-PLA-PEG-Glu-FA
FeCo@SiO2-SS-PEI, acrylate-PLA-PEG-FA and acrylate-PLA- PEG-Glu were dissolved in 6ml of chloroform, separately. The chloroform solution of acrylate-PLA-PEG-FA and acrylate-PLA-PEG-Glu was added dropwise into FeCo@SiO2-SS-PEI solution separately. Then the mixture was stirred for 24 h at 45 °C and stirred for 24 h. The resulting nanoparticles were washed with distilled water and ethanol, then the dispersion was centrifuged at 11068 ×g for 30 minutes and the supernatant was removed. The nanoparticles were lyophilized. To synthesize FeCo@SiO2-SS-PEI-PLA-PEG-Glu-FA nanoparticles, the same amounts of acrylate-PLA-PEG-FA and acrylate-PLA-PEG-Glu were added into FeCo@SiO2-SS-PEI solution. Then the FeCo@SiO2-SS-PEI-PLA-PEG-Glu-FA nanoparticles were synthesized as protocol mentioned above.