Figure Legends
Figure 1: Relatedness of GSU5 with other bacteria.
The 16s rRNA gene of GSU5 was compared against the sequences deposited in the Ribosomal Database Project. A phylogenetic tree was obtained using the Mega 6.0 program. At least one representative of each species of Thermoanaerobacterium , and all strains with sequenced genomes were included, along with the two species of Caldanaerobium , formerly classified within the Thermoanaerobacterium genus.Clostridium acetobutylicum was used as an outgroup.
*Strains with sequenced genomes
Figure 2: Organization of the bcs operon inThermoanaerobacterium thermosaccharolyticum ,Thermoanaerobacterium sp. RBIITD and Clostridium acetobutylicum . Putative Rex operator sites (ROP) are indicated as red circles.
Figure 3: Production of metabolites in T. thermosaccharolyticumGSU5 (A) and T. thermosaccharolyticum DSM 571 (B). Strains were grown in TCS supplemented with different carbon sources in 5 ml Hungate tubes under anaerobic conditions for 48 h. Results represent the mean value ± standard deviation of three independent replicates.
Figure 4: Production of metabolites in T. thermosaccharolyticumGSU5 grown in TCS supplemented with glucose (A) or xylose (B) at 10 g/L in a bioreactor under anaerobic conditions for 36 h.
Data from a representative fermentation of three independent replicates is presented.
Figure 5: Relative distribution (%) of metabolites produced by T. thermosaccharolyticum GSU5 in Hungate tubes with glucose (A), in a bioreactor with glucose (B), in Hungate tubes with xylose (C), and in a bioreactor with xylose (D).
Figure 6: Proposed main metabolic pathways in T. thermosaccharolyticum.
Purple solid arrows indicate reactions catalyzed by genes present in the genome. Red dotted arrows indicate reactions catalyzed by genes absent in the genome. Enzymes separated by a slash and/or with asterisks indicate reactions that could be potentially catalyzed by more than one enzyme. Enzyme names are: Crt: crotonase; Bcd: butyryl-CoA dehydrogenase; EtfAB: electron transfer flavoprotein; Hbd: 3-hydroxybutyryl-CoA dehydrogenase; Thl: acetyl-CoA C-acetyltransferase; But: butyryl-CoA: acetate-CoA transferase; Ptb: phosphotrans butyrylase; Buk: butyrate kinase; Ald: aldehyde dehydrogenase; Bdh: butanol dehydrogenase; AdhE: bifunctional aldehyde/ alcohol dehydrogenase; Pta: phosphotrans acetylase; Ak: acetate kinase; CtfAB: acetoacetyl coenzyme A:acetate/butyrate:coenzyme A transferase; Adc: acetoacetate decarboxylase; Fd red: reduced ferredoxin; Fd ox: oxidized ferredoxin.