Sequence analysis
The sequence was obtained using a whole-genome shotgun strategy with a
Roche 454 GS FLX Titanium pyrosequencer at INDEAR, Argentina, achieving
~37-fold coverage. Assembly was done using Newbler
version 2.6 and generated 73 contigs, the largest of which has 81,110
bases. The annotation was performed using the NCBI Prokaryotic Genomes
Automatic Annotation Pipeline (PGAAP) and Rapid Annotations using
Subsystems Technology (RAST) (Overbeek et al., 2014). The genome of GSU5
was deposited in Genbank with the accession number
MINB01000001-MINB01000073.
Genome to genome distance calculations were performed using the GGDC
program (https://ggdc.dsmz.de/), that provides an in silico
estimation of DNA-DNA hybridization values (Auch, Klenk, & Göker, 2010)
and Average Nucleotide Identity based on blast (ANIb)
http://jspecies.ribohost.com/ jspeciesws (Richter, Rosselló-Móra, Oliver
Glöckner, & Peplies, 2016).