SDS-PAGE and Microchip Analysis
Each sample preparation was described in the Experimental section in the Supporting Information. Samples (0.8 mg/mL, 30 µL) were denatured at 100°C for 3 min after mixing with reducing buffers. Separation took place at 100 V for 30 min within the stacking gel, and 130 V for 2 h within the 12% TRIS-glycine mini gel. Coomassie Blue staining was used for protein visualization, and quantitative microchip analysis based on protein size was performed using a Protein 230 kit (Agilent Technologies, Waldbronn, Germany) following the manufacturer’s protocol on an Agilent 2100 Bioanalyzer (Agilent Technologies).