Exoglycosidase digestion sequencing of the R27T N-glycan by
HILIC-HPLC Arrows indicate digestion. The following exoglycosidases
were used: sialidase (a368S; specific for α2-3, -6, -8, and -9 sialic
acids); beta-galactosidase (b4G; specific for β1-4 galactose);
α-galactosidase (a36G; specific for α1-3/6 galactose); fucosidase (a34F;
specific for α1-3 and -4 fucose); fucosidase (a6F; specific for
α1-6>2 fucose); N -acetylglucosaminidase (sph;
specific for β-GlcNAc). Glycan structures were allocated by a
combination of elution position (expressed as GU value) and subsequent
GU values of peaks following digestion with specific exoglycosidases. *
Please see Structure abbreviations section, Supporting Information.