Experimental Section
Think of this as a recipe cook book, the aim here is to enable the reader to recreate the experiment with the same outcome. How you did it, what materials, tools and techniques you used, Sample preparation, times, temperatures etc. The Experimental section should be precise, and give all details necessary to repeat the work.
materials and methods
https://journal.chestnet.org/article/S0012-3692(15)48992-5/fulltext
For solution-state NMR the relevant ASTM Standard, ASTM E386 - 90(2004), should be followed (see www.astm.org). Where new compounds are discussed, they must be fully characterized in the normal manner including spectral data other than NMR. In the case of natural products the source (e.g. the organism), the isolation procedure and a specimen deposit should be noted. For NMR spectra, all shifts should be given on the delta scale in ppm, high-frequency shifts being denoted as more positive values. Both 1H and 13C shifts should be referenced to internal tetramethylsilane (TMS). Isotope shifts may be given in ppb. All J values should be expressed in Hz. The following spectral details should be provided: nucleus and frequency (MHz); instrument; solvent and concentration (mg ml−1 or mmol ml−1); reference standard; temperature; pulse conditions; computer processing techniques; accuracy of parameters. Where spectral data are reported, the digital resolution must be cited, preferable in terms of spectral widths and the sizes of the data tables. Routine two-dimensional experiments should be described by literature references as far as possible and/or by referring to standard pulse sequences and parameters provided by the spectrometer manufacturers. Where novel two-dimensional techniques are used the following details should be provided: pulse sequence including all flip angles, all delay values, and full phase cycling (preferably in tabular form); spectral widths in f1 and f2; number of t1 increments; number of data points measured per increment; number of transients acquired per increment; weighting functions (if any) in t1 and t2; data matrix size after Fourier transformation; data presentation mode (phase sensitive, absolute value, etc.); total duration of experiment. Further details should be given where appropriate. For continuous wave EPR spectra the following details should be provided: frequency/band; instrument; field range/centre field; field sweep rate; sample phase or solvent used; temperature; modulation amplitude; modulation frequency; microwave power. For pulse EPR the relevant additional parameters should be given. Where EPR spin-Hamiltonian parameters are extracted from spectra by simulation methods, the software used should be specified, along with the parameters, such as linewidths and lineshapes, used in the analysis. Relevant papers in recent issues of the journal should be inspected in case of doubt.