Finally, the new mutated PAP1 fragment is inserted in pGEMTeasy plasmid, allowing amplification of the fragment in bacterial cultures. A correct insertion is curial. The plasmid contains an antibiotic resistance gene allowing bacteria that have incorporated the plasmid to survive on ampicillin medium : we can then discriminate the colonies with the correctly transformed plasmid by seeing which ones are white instead of blue. The plasmid also contains a lacZ gene with the insertion box right in the middle ; the insertion of the PAP1 fragment will then cut this gene making its product non functional. As a result, there is no degradation of X-Gal, and no blue product produced (Fig. 1).
Conclusion
In progress
Acknowledgements
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