Figure 3. (a) Illustration of dendrimersome-NTA-His-SNAP-BG-ALC aptamer for loading fluorescent DNA on the surface of dendrimersomes. (b) Illustration of NTA-His-SNAP-BG-ALC aptamer. (c) Dendrimersomes consiting of 5% (mg/mL) RH-NTA and 95% (mg/mL) RH-10 dendrimer are more amenable to tether an Anti Lung Carcinoma (ALC) aptamer (5’-NHS-BG-ALC-3’-FAM) than stealth liposome containing 5% (mg/mL) of RH-NTA. The tethering on the membrane of these vesicles occurs through the binding of the BG-NHS group (at 5’ end) with a GFPb-SNAP-6His (1mM) protein used during the swelling of the vesicles. The GFPb-SNAP-6His tethers the RH-NTA dendrimer or the DGS-NTA lipid on the membrane of the dendrimersomes and the liposomes. Representative confocal images of stealth liposomes, containing DGS-NTA and 18:1 Rho-PE lipid, used to stain the membrane (red). (d) The graph corresponds to the quantification of the ring intensity of the ALC apatmer tethered to the lamellae of dendrimersomes and stealth liposome.