Figure 2. Dendrimersomes are more amenable than Stealth Liposomes for loading 6-HisGFP. The addition of RH-NTA leads to tethering of 6-His-GFP on the lamellae of unilamellar Dendrimersomes. In contrast Onion Dendrimersomes present an outer coating of 6-His GFP protein, on the outer lamellae. The histogram (a) shows that dendrimersomes consisting of RH-10 and RH-12, both unilamellar and both onions compared with unilamellar and multlamellar stealth liposomes. The histogram (b) demonstartes that in presence of RH-NTA, the fluorescence intensity increased dramatically compared the ones without RH-NTA. The histogram (c) demonstartes that in presence of RH-NTA, the fluorescence intensity of the ring, corresponding to the tethering of 6-HisGFP at the boundary, is higher for unilamellar vesicles than for onions. (d–e) C images of onions and unilamellar vesicles (d) without and (e) with RH-NTA.