This study aims to implement high resolution T1 maps based on 3D fast low angle shot (FLASH) imaging and measure the values between different tissue types in a whole brain. A series of 3D FLASH images were acquired with three different tip angles and the variation was evaluated on the brain tissue types. The T1 maps and relative Proton Density (PD) images with these tip angles were calculated and shown in all directions. Also the values between similar tissues at different regions at which located the white matter in corpus callosum and gray matter in the putamen were compared with the approximate general values known as the literature.