which is the difference between the number of electrons per carbon in the final reduced biofuel, \(n_{ef}\), and the number of electrons per carbon in the primary fixation product, \(n_{ep}\), multiplied by the ratio of carbons in the fuel and the primary fixation product, \(\frac{n_{Cf}}{n_{Cp}}\).
2- Results and Discussion
2.1- Internal parameters consideration
2.1.1-Menaquinone and CytochromeC potential
For EET-mediated hybrid photosynthesis, many variables were investigated. In the case of EET-mediated microbes, the issue lies in the inability for menaquinone to reduce NAD, unlike \(H_2\). To promote electrons to reduce NADH some electrons from menaquinone in the membrane are used to reduce \(O_2\) into the water to provide the necessary energy for other electrons in other molecules of menaquinone.
Therefore, the effect of menaquinone potential in different membrane potential \(\Delta V_{membrane}\), where the potential outside the cell is measured with respect to the potential inside the cell, on thermionic efficiency was studied (Figure 3A).
This allows us to determine the fraction of electrons on menaquinone that will be promoted to reduce NAD (Figure 2B). The electron transfers were assumed to be multi-electron transfers that would simultaneously transfer all required electrons to the acceptor molecules, as this minimized any inefficiencies associated with equations (2) and (3).
Some incoming electrons are sent downhill in energy to a terminal electron acceptor, pumping protons against the gradient, and adding to a reservoir of stored energy that can be used to send some electrons uphill.
The number of protons that can be pumped up against the proton gradient by sending an electron downhill,